Recombinant Human Flt-3 Ligand/FLT3L (E. coli) Protein, CF Best Seller

Catalog # 308-FKE | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

P49771.1

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human Flt-3 Ligand/FLT3L protein
Thr27-Ala181

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met and Thr27

Predicted Molecular Mass

18 kDa

SDS-PAGE

15-17 kDa, under reducing conditions

Activity

Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3.
The ED50 for this effect is 0.2-1 ng/mL.

Formulation, Preparation and Storage

308-FKE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100-500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3 Ligand/FLT3L

Flt‑3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature human Flt‑3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail (4-7). Within the ECD, human Flt‑3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt‑3 Ligand, respectively (4-6). The human and mouse Flt‑3 Ligand proteins show cross-species activity. Flt-3 Ligand is also structurally related to M-CSF and SCF. Flt-3 Ligand is widely expressed in various human and mouse tissues. It is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O-linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain (4, 8). Alternate splicing of human Flt‑3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine-like domain in the ECD of the Flt-3 Ligand protein (4, 5, 8). Both transmembrane and soluble forms of Flt‑3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 4, 6, 7). Flt‑3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 9). Additionally, Flt-3 Ligand synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4-6). Flt-3 Ligand also cooperates with IL-2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL-11 to induce terminal B cell maturation (1, 10). Animal studies show that Flt‑3 Ligand reduces the severity of experimentally induced allergic inflammation (11).

References

  1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
  2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
  3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
  4. Hannum, C. et al. (1994) Nature 368:643.
  5. Lyman, S.D. et al. (1994) Blood 83:2795.
  6. Lyman, S.D. et al. (1993) Cell 75:1157.
  7. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
  8. McClanahan, T. et al. (1996) Blood 88:3371.
  9. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
  10. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
  11. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Entrez Gene IDs

2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)

Gene Symbol

FLT3LG

UniProt

P49771.1

Product Documents for Recombinant Human Flt-3 Ligand/FLT3L (E. coli) Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Flt-3 Ligand/FLT3L (E. coli) Protein, CF

For research use only

Citations for Recombinant Human Flt-3 Ligand/FLT3L (E. coli) Protein, CF (2)

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FAQs for Recombinant Human Flt-3 Ligand/FLT3L (E. coli) Protein, CF

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  • Q: What are the differences between Recombinant Human Flt-3 Ligand/FLT3L Protein (Catalog # 308-FK), Recombinant Human Flt-3 Ligand/FLT3L Protein (Catalog # 308-FKN), and Recombinant Human Flt-3 Ligand/FLT3L Protein, CF (Catalog # 308-FKE)?

    A: Recombinant Human Flt-3 Ligand/FLT3L Protein (Catalog # 308-FK) and Recombinant Human Flt-3 Ligand/FLT3L Protein (Catalog # 308-FKN) share the same protein sequence, Thr27-Pro185 of Accession # AAA17999.1 but Catalog # 308-FK was expressed in Sf 21 baculovirus cells and Catalog # 308-FKN was produced in mammalian NS0 cells. Both of these proteins are glycosylated and available bottled with BSA or without BSA (Carrier-Free).  Recombinant Human Flt-3 Ligand/FLT3L Protein, CF (Catalog # 308-FKE) was produced in an E. Coli expression system and has a sequence of (Thr27-Ala181, Accession # P49771.1), but may also contain an N-terminal Methionine followed by the expected sequence. In addition to being slightly shorter compared to 308-FKN and 308-FK, there is one difference in amino acid at position 72. Accession # AAA17999.1 has an Alanine at position 72, while Accession # P49771.1 has a Glycine in that position. All three versions of Recombinant Human Flt-3 Ligand/FLT3L proteins are routinely tested and have the same activity range in our QC testing assay: Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.  R&D Systems also offers two GMP versions of this protein, an E. coli-derived animal-free version, Recombinant Human Flt-3 Ligand/FLT3L GMP Protein, CF (Catalog # 308E-GMP), and an Sf-21 baculovirus-derived version, Recombinant Human Flt-3 Ligand GMP Protein, CF (Catalog # 308-GMP). For information on the GMP proteins, we recommend consulting the product-specific pages for each protein. To determine which of these three proteins is most suitable for an application, we would recommend checking the Citations tab on the product-specific page.

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