Recombinant Human Flt-3 Ligand/FLT3L Animal-Free Protein
R&D Systems, part of Bio-Techne | Catalog # AFL308E

Discontinued Product
AFL308E has been discontinued.
An alternative/replacement product is available:
BT-FT3L-AFL. View all Flt-3 Ligand/FLT3L products.
Key Product Details
Learn more about Animal-Free Recombinant Proteins
Product Specifications
Source
E. coli-derived human Flt-3 Ligand/FLT3L protein
Thr27-Ala181
Produced using non-animal reagents in an animal-free laboratory.
Thr27-Ala181
Produced using non-animal reagents in an animal-free laboratory.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met and Thr27
Predicted Molecular Mass
18 kDa
SDS-PAGE
15-17 kDa, under reducing conditions
Activity
Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3.
The ED50 for this effect is 0.200-1.00 ng/mL.
The ED50 for this effect is 0.200-1.00 ng/mL.
The specific activity of Recombinant Human Flt-3 Ligand/FLT3L is >5.00 x 105 units/mg, which is calibrated against the human Flt-3 Ligand WHO Standard (NIBSC code: 96/532).
Formulation, Preparation and Storage
AFL308E
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100-500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Flt-3 Ligand/FLT3L
References
- Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
- Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
- Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
- Hannum, C. et al. (1994) Nature 368:643.
- Lyman, S.D. et al. (1994) Blood 83:2795.
- Lyman, S.D. et al. (1993) Cell 75:1157.
- Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
- McClanahan, T. et al. (1996) Blood 88:3371.
- Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
- Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
- Edwan, J.H. et al. (2004) J. Immunol. 172:5016.
Long Name
fms-like Tyrosine Kinase 3 Ligand
Alternate Names
FLG3L, Flt3L, FLT3LG
Gene Symbol
FLT3LG
UniProt
Additional Flt-3 Ligand/FLT3L Products
Product Documents for Recombinant Human Flt-3 Ligand/FLT3L Animal-Free Protein
Manufacturing Specifications
Animal-Free Manufacturing ConditionsOur dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.
Production
- All molecular biology procedures use animal-free media and dedicated labware.
- Dedicated fermentors are utilized in committed animal-free areas.
Purification
- Protein purification columns are animal-free.
- Bulk proteins are filtered using animal-free filters.
- Purified proteins are stored in animal-free containers in a dedicated cold storage room.
Quality Assurance
- Low Endotoxin Level.
- No impairment of biological activity.
- High quality product obtained under stringent conditions.
- For ex vivo research or bioproduction, additional documentation can be provided.
Product Specific Notices for Recombinant Human Flt-3 Ligand/FLT3L Animal-Free Protein
For research use only
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