Source	Mouse myeloma cell line, NS0-derived human FCRL3/FcRH3 protein Arg14-Arg569, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Arg14
Predicted Molecular Mass	62.2 kDa
SDS-PAGE	80-90 kDa, reducing conditions
Activity	Measured by its ability to bind human IgG with an estimated K_D <60 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3126-FC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FCRL3/FcRH3

FCRL3 (Fc Receptor-Like 3), also known as FcRH3, IRTA3, and SPAP2, is a 110 kDa molecule with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1). Mature human FCRL3 consists of a 556 amino acid (aa) extracellular domain (ECD) with six Ig-like domains, a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain with four immunotyrosine inhibitory motifs (ITIMs) (2 - 4). Within the ECD, human and mouse FCRL3 share 35% aa sequence identity. Alternate splicing generates several additional isoforms with deletions or substitutions in both the extracellular and intracellular regions. These include potentially secreted forms that are truncated following the second Ig-like domain (4). FCRL3 is expressed in secondary lymphoid organs on the surface of mature naïve and memory B cells, NK cells, and B cell lines derived from chronic lymphocytic leukemias (2, 3, 5). It is upregulated on B cells following LPS or anti-CD40 stimulation (6). A polymorphism in the FCRL3 promoter induces enhanced transcription and is associated with the development of autoimmune disorders in a Japanese population (6, 7). Tyrosine phosphorylation within the ITIMs of FCRL3 enables its association with SHP-1 (4).

Item	Value
References	Davis, R.S. (2007) Annu. Rev. Immunol. 25:525. Miller, I. et al. (2002) Blood, 99:2662. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772. Xu, M.-J. et al. (2002) Biochem. Biophys. Res. Commun. 293:1037. Polson, A.G. et al. (2006) Int. Immunol. 18:1363. Kochi, Y. et al. (2005) Nat. Genet. 37:478. Chistiakov, D.A. and A.P. Chistiakov (2007) Hum. Immunol. 68:375.
Long Name	Fc Receptor-like 3
Entrez Gene IDs	115352 (Human); 329693 (Mouse); 680697 (Rat)
Alternate Names	CD307c, FCRL3, Fc receptor homolog 3, Fc receptor-like 3, Fc receptor-like protein 3, FcRH3, IFGP3, IRTA3, SPAP2, fcR-like protein 3, hIFGP3, immunoglobulin superfamily receptor translocation associated protein 3

Citations for Recombinant Human FCRL3/FcRH3 Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human FCRL3/FcRH3 Protein, CF include:

Species: Human
Sample Types: Protein
Applications: Surface Plasmon Resonance (SPR

S Agarwal et al. (2020), Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA Cell Rep, 30(5):1292-1299.e3.
PMID: 32023449
Species: Human
Sample Types: Recombinant Proteins
Applications: Surface Plasmon Resonance (SPR

Franco A et al. (0), Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors. J Immuno190(11):5739-46.
PMID: 23616577

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Customer Reviews for Recombinant Human FCRL3/FcRH3 Protein, CF (2)

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Recombinant Human FCRL3/FcRH3 Protein, CF

Name: Gerre Lafond

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 02/19/2021
Recombinant Human FCRL3/FcRH3 Protein, CF

Name: Alisha Freeman

Application: Enzymatic activity in vitro

Verified Customer | Posted 10/09/2020

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Product Documents for Recombinant Human FCRL3/FcRH3 Protein, CF

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Recombinant Human FCRL3/FcRH3 Protein, CF

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Product Specifications for Recombinant Human FCRL3/FcRH3 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: FCRL3/FcRH3

Citations for Recombinant Human FCRL3/FcRH3 Protein, CF (2)

Customer Reviews for Recombinant Human FCRL3/FcRH3 Protein, CF (2)

Recombinant Human FCRL3/FcRH3 Protein, CF

Recombinant Human FCRL3/FcRH3 Protein, CF

Product Documents for Recombinant Human FCRL3/FcRH3 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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