Recombinant Human Calcineurin Protein, CF

Name: Recombinant Human Calcineurin Protein, CF
Brand: R&D Systems
SKU: 3160-CA

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3160-CA

Citations (3)

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Source:	Sf 21 (baculovirus)
Accession #:	NP_000935 (Calcineurin A) NP_000936 (Calcineurin B
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human Calcineurin Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Calcineurin protein

Human Calcineurin A
(Ser2-Gln521)
Accession # NP_000935

Human Calcineurin B (Met1-Val170) Accession # NP_000936
N-terminus	C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asp15 (Calcineurin A) & Asp17 (Calcineurin B)

Predicted Molecular Mass

57 kDa (Calcineurin A), 18 kDa (Calcineurin B)

SDS-PAGE

43-65 kDa & 15-20 kDa, reducing conditions

Activity

Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO₃)VAAE(Catalog # ES012). The specific activity is >900 nmol/min/mg, under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3160-CA

Formulation	Supplied as a 0.2 μm filtered solution in HEPES, CaCl₂, MgCl₂, DTT and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

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Background: Calcineurin

Calcineurin, also called Protein Phosphatase 2B, PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59,000 dalton catalytic A subunit and a 19,000 dalton regulatory B subunit that is activated by the binding of calcium ions and calmodulin (1). Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory (2). It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation (3). Complexes of the immunosuppressants cyclosporin and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity (4). Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy (5) and graft versus host disease in organ transplantation (6).

Item	Value
References	Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859. Zeng H. et al. (2001) Cell 107:617. Okamura, H. et al. (2000) Molecular Cell 6:539. Liu, J. et al. (1992) Biochemistry 31:3896. Molkentin, J.D. (2000) Circulation Research 87:731. Sanquer, S.A. et al. (2004) Transplantation 77:854.
Entrez Gene IDs	5530 (Human)
Alternate Names	CALN, Calcineurin

Citations for Recombinant Human Calcineurin Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Calcineurin Protein, CF include:

Species: Human
Sample Types: Revombinant Protein
Applications: Bioassay

T Goshima et al. (2019), Calcineurin regulates cyclin D1 stability through dephosphorylation at T286 Sci Rep, 9(1):12779.
PMID: 31484966
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Recruitment of calcineurin to the TCR positively regulates T cell activation Nat. Immunol, 0(0).
PMID: 27941787
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Duan L et al. (2010), Calcineurin increases glucose activation of ERK1/2 by reversing negative feedback. Proc. Natl. Acad. Sci. U.S.A., 107(51):22314-9.
PMID: 21135229

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Showing 1 - 3 of 3 citations Showing All

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Recombinant Human Calcineurin Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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