Recombinant Human Ataxin-3 Like Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E-343

R&D Systems, part of Bio-Techne
Discontinued Product
E-343 has been discontinued. View all Ataxin-3 Like products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

ATXN3L

Conjugate

Unconjugated

Applications

Bioactivity

View all Ataxin-3 Like Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ataxin-3 Like protein
Contains a C-terminal 6His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

42 kDa

Activity

Recombinant Human His6-Ataxin-3-Like protein is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial His6-Ataxin-3-like concentration of 10-100 nM when utilizing K63-linked tetra-Ubiquitin Rhodamine (UC-355).  Higher concentrations may be required for other substrates.

Formulation, Preparation and Storage

E-343
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Ataxin-3 Like

Ataxin-3-Like protein, also known as Machado-Joseph Disease (MJD) Protein-1-Like, is a 355 amino acid deubiquitinating (DUB) enzyme with a predicted molecular weight of 41 kDa.  Full-length Ataxin-3-L contains an N-terminal Joesephin Domain, two Ubiquitin-Interacting-Motifs (UIM's) and a C-terminus consisting of a polyglutamine stretch and tail.  Ataxin-3-L targets include Kruppel-Like Factor 5 (KLF5).  KLF5 has been reported to promote breast cell proliferation, survival and tumorigenesis.  Ubiquitination and proteasome-mediated degradation of KLF5 is antagonized by Ataxin-3-L, suggesting that inhibition of this DUB might provide a target for therapeutic intervention in breast cancer.  Ataxin-3 and Ataxin-3-L have been reported to cleave both K48- and K63-linked poly-Ubiquitin in vitro, though enzyme activity in such assays is inefficient when using shorter chain lengths (≤ 6).  Ataxin-3-L activity can be efficiently monitored with K63-linked tetra-Ubiquitin Rhodamine (http://www.rndsystems.com/product_results.aspx?k=UC-355">UC-355) substrate.

References

  1. Albrecht, M. et al. (2004) Eur. J. Biochem.   271: 3155
  2. Bettencourt, C. & M. Lima (2011) Orphanet. J. Rare Dis.  6: 35
  3. Ge, F. et al. (2015)  Oncotarget  6: 21369
  4. Giuseppe, N. et al.  (2010)  PLoS   5: 10.1371/journal.pone.0012430
  5. Rodrigues, A.J. et al. (2007)  FASEB J.  21: 1126
  6. Scaglione, K.M. et al. (2011)  Mol. Cell.  43: 599
  7. Todi, S.V. et al. (2009)  EMBO J.  28: 372
 

Alternate Names

Ataxin 3 Like, Ataxin-3-like, ATX3L, ATXN3L, MJDL

Entrez Gene IDs

92552 (Human)

Gene Symbol

ATXN3L

UniProt

ATXN3L

Additional Ataxin-3 Like Products

Product Documents for Recombinant Human Ataxin-3 Like Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Ataxin-3 Like Protein, CF

For research use only

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