ProDots Recombinant Human TGF-beta 1 Protein
R&D Systems, part of Bio-Techne | Catalog # PRD240
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD240 has been discontinued.
View all TGF-beta 1 products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein
Ala279 - Ser390
Ala279 - Ser390
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala279
Predicted Molecular Mass
12.8 kDa (monomer)
SDS-PAGE
12 kDa, reducing conditions, 24 kDa, non-reducing conditions
Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is 0.04-0.2 ng/mL.
The ED50 for this effect is 0.04-0.2 ng/mL.
Scientific Data Images for ProDots Recombinant Human TGF-beta 1 Protein
ProDots Recombinant Human TGF-beta 1 Protein Bioactivity
ProDots®Recombinant Human TGF-beta 1 inhibits Recombinant Mouse IL-4 (R&D Systems, Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED50 is 0.04-0.2 ng/mL.
ProDots Recombinant Human TGF-beta 1 Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human TGF-beta 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing single bands at 12 kDa and 24 kDa, respectively.Formulation, Preparation and Storage
PRD240
| Reconstitution | For a stock solution, reconstitute at 20 μg/mL in sterile 4 mM HCl, or simply roll ProDot® directly into cell culture medium for immediate use.
Note: Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Background: TGF-beta 1
TGF-beta 1 (Transforming Growth Factor beta 1) is a pleiotrophic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. TGF-beta 1 associates with LAP to form a latent complex. It can be is activated from latency by plasmin, matrix metalloproteases, thrombospondin 1, and a subset of integrins. TGF-beta 1 signals through complexes of TGF-beta RII with TGF-beta RI/ALK-5 or ALK-1. TGF-beta signaling is modulated by the accessory receptors TGF-beta RIII/Betaglycan and Endoglin/CD105.
Long Name
Transforming Growth Factor beta 1
Alternate Names
TGF beta1, TGFB, TGFB1, TGFbeta 1
Gene Symbol
TGFB1
UniProt
Additional TGF-beta 1 Products
Product Documents for ProDots Recombinant Human TGF-beta 1 Protein
Product Specific Notices for ProDots Recombinant Human TGF-beta 1 Protein
726377.4
For research use only
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