ProDots Recombinant Human SCF/c-kit Ligand Protein
R&D Systems, part of Bio-Techne | Catalog # PRD255
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD255 has been discontinued.
View all SCF/c-kit Ligand products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Product Specifications
Source
E. coli-derived human SCF/c-kit Ligand protein
Glu26-Ala189, with an N-terminal Met
Glu26-Ala189, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
18.6 kDa
SDS-PAGE
19 kDa, reducing conditions.
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 1-5 ng/mL.
The ED50 for this effect is 1-5 ng/mL.
Scientific Data Images for ProDots Recombinant Human SCF/c-kit Ligand Protein
ProDots Recombinant Human SCF/c-kit Ligand Protein Bioactivity
ProDots®Recombinant Human SCF stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 1-5 ng/mL.
ProDots Recombinant Human SCF/c-kit Ligand Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human SCF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 19 kDa.Formulation, Preparation and Storage
PRD255
| Reconstitution | For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Background: SCF/c-kit Ligand
SCF (Stem cell factor), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF) promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. SCF is expressed as a transmembrane, but a soluble form can be released by proteolytic shedding. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
Long Name
Stem Cell Factor
Alternate Names
c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF
Gene Symbol
KITLG
UniProt
Additional SCF/c-kit Ligand Products
Product Documents for ProDots Recombinant Human SCF/c-kit Ligand Protein
Product Specific Notices for ProDots Recombinant Human SCF/c-kit Ligand Protein
726192.5
For research use only
Loading...
Loading...
Loading...