ProDots Recombinant Human SCF/c-kit Ligand Protein

R&D Systems, part of Bio-Techne | Catalog # PRD255

Supplied as ready-to-use 5 ug protein balls
R&D Systems, part of Bio-Techne
Discontinued Product
PRD255 has been discontinued. View all SCF/c-kit Ligand products.

Key Product Details

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Features and Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Source

E. coli

Accession #

P21583.1

Conjugate

Unconjugated

Applications

Bioactivity

View all SCF/c-kit Ligand Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human SCF/c-kit Ligand protein
Glu26-Ala189, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

18.6 kDa

SDS-PAGE

19 kDa, reducing conditions.

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 1-5 ng/mL.

Scientific Data Images for ProDots Recombinant Human SCF/c-kit Ligand Protein

ProDots Recombinant Human SCF/c-kit Ligand Protein Bioactivity

ProDots Recombinant Human SCF/c-kit Ligand Protein Bioactivity

ProDots®Recombinant Human SCF stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 1-5 ng/mL.
ProDots Recombinant Human SCF/c-kit Ligand Protein SDS-PAGE

ProDots Recombinant Human SCF/c-kit Ligand Protein SDS-PAGE

1 μg/lane of ProDots®Recombinant Human SCF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 19 kDa.

Formulation, Preparation and Storage

PRD255
Reconstitution For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied.
• 12 months from date of receipt at 2-8 °C as supplied.
• 1 month at 2-8 °C under sterile conditions after reconstitution.
• 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75 - 83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF-R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

Long Name

Stem Cell Factor

Alternate Names

c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

Gene Symbol

KITLG

UniProt

P21583.1

Additional SCF/c-kit Ligand Products

Product Documents for ProDots Recombinant Human SCF/c-kit Ligand Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for ProDots Recombinant Human SCF/c-kit Ligand Protein

726192.5

For research use only

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