ProDots Recombinant Human Noggin Protein
R&D Systems, part of Bio-Techne | Catalog # PRD6057
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD6057 has been discontinued.
View all Noggin products.
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Noggin protein
Gln28 - Cys232
Gln28 - Cys232
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln28 predicted
Predicted Molecular Mass
23 kDa (monomer)
SDS-PAGE
30-33 kDa, reducing conditions
Activity
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
The ED50 for this effect is typically 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).
The ED50 for this effect is typically 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).
Scientific Data Images for ProDots Recombinant Human Noggin Protein
ProDots Recombinant Human Noggin Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human Noggin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 30 kDa and 150 kDa, respectively.
ProDots Recombinant Human Noggin Protein Bioactivity
ProDots®Recombinant Human Noggin inhibits BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).Formulation, Preparation and Storage
PRD6057
| Reconstitution | For a stock solution, reconstitute at 250 μg/mL in sterile PBS. For immediate use, simply roll ProDot® directly into cell culture medium. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Background: Noggin
Alternate Names
NOG, SYM1, SYNS1, SYNS1A
Gene Symbol
NOG
UniProt
Additional Noggin Products
Product Documents for ProDots Recombinant Human Noggin Protein
Product Specific Notices for ProDots Recombinant Human Noggin Protein
727086.0
For research use only
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