ProDots Recombinant Human IL-6 Protein
R&D Systems, part of Bio-Techne | Catalog # PRD206
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD206 has been discontinued.
View all IL-6 products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Product Specifications
Source
E. coli-derived human IL-6 protein
Pro29 - Met212
Pro29 - Met212
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Pro29
Predicted Molecular Mass
20.3 kDa
SDS-PAGE
21 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813.
The ED50 for this effect is 0.2-0.8 ng/mL.
Scientific Data Images for ProDots Recombinant Human IL-6 Protein
ProDots Recombinant Human IL-6 Protein Bioactivity
ProDots®Recombinant Human IL-6 stimulates cell proliferation of the T1165.85.2.1 mouse plasmacytoma cell line.The ED50 for this effect is 0.2-0.8 ng/mL.
ProDots Recombinant Human IL-6 Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human IL-6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 21 and 20 kDa, respectively.Formulation, Preparation and Storage
PRD206
| Reconstitution | For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual
defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
R&D Systems, a Bio-Techne brand, offers a comprehensive range of IL-6 Antibody products, IL-6 Proteins and Enzymes, IL-6 ELISAs, IL-6 Luminex Assays and more. Use the filters above to drill down to the IL-6 product that best fits your needs.
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Entrez Gene IDs
Gene Symbol
IL6
UniProt
Additional IL-6 Products
Product Documents for ProDots Recombinant Human IL-6 Protein
Product Specific Notices for ProDots Recombinant Human IL-6 Protein
Part # 726221.1
For research use only
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