ProDots Recombinant Human Flt-3 Ligand/FLT3L Protein

Name: ProDots Recombinant Human Flt-3 Ligand/FLT3L Protein
Brand: R&D Systems
SKU: PRD308

R&D Systems, Inc. a Bio-Techne Brand

Catalog # PRD308

Product Datasheet / COA / SDS

Supplied as ready-to-use 5 ug protein balls

Discontinued Product

PRD308 has been discontinued. View all Flt-3 Ligand/FLT3L products.

Setting the standard in quality research reagents

Key Product Details

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Features and Benefits

Rolls out of bottle for easy cell culture medium preparation
Eliminates time spent aliquoting
One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Source:	Sf 21 (baculovirus)
Accession #:	AAA17999.1
Applications:	Bioactivity

View Full Technical Details for ProDots Recombinant Human Flt-3 Ligand/FLT3L Protein

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Product Specifications for ProDots Recombinant Human Flt-3 Ligand/FLT3L Protein

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Flt-3 Ligand/FLT3L protein Thr27-Pro185
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Thr27
Predicted Molecular Mass	17.5 kDa
SDS-PAGE	17-30 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED₅₀for this effect is 0.2-1 ng/mL.

Scientific Data Examples for ProDots Recombinant Human Flt-3 Ligand/FLT3L Protein

	ProDots Recombinant Human Flt-3 Ligand/FLT3L Protein Bioactivity ProDots^®Recombinant Human Flt-3 Ligand stimulates cell proliferation in the BaF3 mouse Pro-B cell line transfected with mouse Flt-3. The ED₅₀ for this effect is 0.2-1 ng/mL.
	ProDots Recombinant Human Flt-3 Ligand/FLT3L Protein SDS-PAGE 1 μg/lane of ProDots^®Recombinant Human Flt-3 Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized with silver staining, showing major bands at 20-24 kDa. The multiple bands are due to variable glycosylation of the protein.

Formulation, Preparation and Storage

PRD308

Reconstitution	For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot^® directly into cell culture medium for immediate use. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Background: Flt-3 Ligand/FLT3L

Flt‑3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature human Flt‑3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail (4-7). Within the ECD, human Flt‑3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt‑3 Ligand, respectively (4-6). The human and mouse Flt‑3 Ligand proteins show cross-species activity. Flt-3 Ligand is also structurally related to M-CSF and SCF. Flt-3 Ligand is widely expressed in various human and mouse tissues. It is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O-linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain (4, 8). Alternate splicing of human Flt‑3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine-like domain in the ECD of the Flt-3 Ligand protein (4, 5, 8). Both transmembrane and soluble forms of Flt‑3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 4, 6, 7). Flt‑3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 9). Additionally, Flt-3 Ligand synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4-6). Flt-3 Ligand also cooperates with IL-2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL-11 to induce terminal B cell maturation (1, 10). Animal studies show that Flt‑3 Ligand reduces the severity of experimentally induced allergic inflammation (11).

Item	Value
References	Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532. Hannum, C. et al. (1994) Nature 368:643. Lyman, S.D. et al. (1994) Blood 83:2795. Lyman, S.D. et al. (1993) Cell 75:1157. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486. McClanahan, T. et al. (1996) Blood 88:3371. Diener, K.R. et al. (2008) Exp. Hematol. 36:51. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.
Long Name	fms-like Tyrosine Kinase 3 Ligand
Entrez Gene IDs	2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)
Alternate Names	FL, FLG3L, FLT3LG, Flt-3 Ligand, Flt3 ligand, Flt3L, SL cytokine, fms-related tyrosine kinase 3 ligand

Product Specific Notices for ProDots Recombinant Human Flt-3 Ligand/FLT3L Protein

726223.1

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