Human Blood B Booster Kit

Dendritics has developed the HuBBB(R) kit as an improvement of the above approaches, in order to target the largest B cell population from peripheral blood, thus offering the opportunity to pursue studies on immortalized B cells representative of the overall peripheral B repertoire. Under the best conditions, the kit allows the immortalization of up to 20% of the starting B cell population. The HuBBB(R) kit was packaged so as to make it very easy to handle for the scientists ; it contains all the reagents needed to obtain from 5.104 to 2.105 independent B cell clones from 107 peripheral blood mononuclear cells using ten 96 wells culture plates. The resulting cells are available for a wide range of studies including genomic, transcriptomic and proteomic fields.

Intended usage: -Production of human monoclonal antibodies for research use. -Drug discovery/development: development of fully human antibodies for use in in vitro studies. -Evaluation of biotherapeutics agents or candidate vacines on immunoglobulin secretion. -Measurment of auto/allo-antibodies against specific biological targets like cytokines. Successful Proofs of Concepts: -Fully human monoclonal antibodies were made against several targets including HuMAB anti-HPA5b, -IL1a, -BetV1, -Derp1 -Neutralizing HumAB anti-Chik -Hu and canMab anti-Leptospira