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Janelia Fluor® 646, SE

Amine-Reactive Janelia Fluor Dyes  Small Molecule
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Description: Red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging
Alternative Names: JF 646, NHS
Chemical Name: 1-[7-(1-Azetidinyl)-10-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt
Molecular Weight: 593.71

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  • JF646 labeled proteins in vitro
    Name: Saumya Saurabh
    Species: Other
    Assay Type: In Vitro
    I used this dye for labeling bacterial proteins in vitro. The labeling method is pretty straight-forward as for any NHS-protein coupling. The dye is highly photostable as determined by single protein tracking on lipid bilayers (image of single proteins attached, scale bar is 5 micron). The only reason I give the product 4 stars instead of 5 is the packaging. The dye is shipped as a solid in a glass bottle with a flexible rubber cap. Most of the dye gets stuck inside and on the surface of this cap; thus not delivering a full value for money. I will highly recommend the manufacturers to use bottles with plastic caps so that the dye can be recovered a 100%
    Janelia Fluor® 646, SE Serum 6148

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