Bis-ANS

Name: Bis-ANS
Brand: Tocris Bioscience
SKU: 5908

Tocris Bioscience, part of Bio-Techne | Catalog # 5908

COA / SDS

Fluorescent probe for nonpolar cavities in proteins; used to detect Aβ fibres

Discontinued Product

5908 has been discontinued. View all Fluorescent Amyloid B Probes products.

Key Product Details

Description:	Fluorescent probe for nonpolar cavities in proteins; used to detect Aβ fibres
Chemical Name:	4,4'-Bis(phenylamino)-[1,1'-binaphthalene]-5,5'-disulfonic acid dipotassium salt
Purity:	≥97% (HPLC)
Molecular Weight:	672.85

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Technical Data
Citations

Biological Activity for Bis-ANS

Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect Aβ fibre (K_d = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4. Also modulates liquid-liquid phase separation (LLPS) of TDP-43 low complexitry domain in vitro. Displays biphasic modulation; promotes LLPS at low concentrations but disprut liquid droplets at higher concentrations.

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Technical Data for Bis-ANS

M. Wt	672.85
Formula	C₃₂H₂₂K₂N₂O₆S₂
Storage	Store at RT
Purity	≥97% (HPLC)
CAS Number	65664-81-5
PubChem ID	16213473
InChi Key	VWLWTJHKQHRTNC-UHFFFAOYSA-L
Smiles	O=S(C1=CC=CC2=C1C(NC3=CC=CC=C3)=CC=C2C4=CC=C(NC5=CC=CC=C5)C6=C(S(=O)([O-])=O)C=CC=C46)([O-])=O.[K+].[K+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for Bis-ANS

References are publications that support the biological activity of the product.

Younan A comparison of three fluorophores for the detection of amyloid fibers and prefibrillar oligomeric assemblies. ThT (Thioflavin T); ANS (1-Anilinonaphthalene-8-sulfonic Acid); and bisANS (4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic Acid). Biochemistry 2015 PMID: 26087242
Babinchak Small molecules as potent biphasic modulators of protein liquid-liquid phase separation Nat.Commun. 2020 PMID: 33149109
Rosen Dimer formation from 1-amino-8-naphthalenesulfonate catalyzed by bovine serum albumin. A new fluorescent molecule with exceptional binding properties. Biochemistry 1969 PMID: 5388144