5(6)-SFX (Fluorescein), SE

Name: 5(6)-SFX (Fluorescein), SE
Brand: Tocris Bioscience
SKU: 6488

Tocris Bioscience, part of Bio-Techne | Catalog # 6488

COA / SDS

Green fluorescent dye for the labeling of primary amines

Discontinued Product

6488 has been discontinued. View all Standard Fluorescein, Coumarin and Rhodamine-based products.

Key Product Details

Description:	Green fluorescent dye for the labeling of primary amines
Chemical Name:	6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester
Purity:	≥90% (HPLC)
Molecular Weight:	586.55

View Full Technical Details for 5(6)-SFX (Fluorescein), SE

Technical Data
Citations

Biological Activity for 5(6)-SFX (Fluorescein), SE

5(6)-SFX (Fluorescein), SE is a green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm^-1M^-1.

Please see the protocol for further information on protein/antibody labeling and conjugation.

Optical Data for 5(6)-SFX (Fluorescein), SE

Emission Color	Green
λ_abs	494 nm
λ_em	518 nm
Extinction Coefficient (ε)	70000 M^-1cm^-1
Closest Laser line	488 nm
Reactive Group	NHS ester
Reactivity	Primary amines
Application	Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for 5(6)-SFX (Fluorescein), SE

M. Wt	586.55
Formula	C₃₁H₂₆N₂O₁₀
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	114616-31-8
PubChem ID	122174028
InChi Key	WAGYELYAQOYZFU-UHFFFAOYSA-N
Smiles	OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)OC(C5=C3C=CC(C(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)=C5)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)OC(C%11=C9C=C(C(NCCCCCC(ON%12C(CCC%12=O)=O)=O)=O)C=C%11)=O)C=C7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for 5(6)-SFX (Fluorescein), SE

References are publications that support the biological activity of the product.

Ito Artificial human Met agonists based on macrocycle scaffolds. Nat.Commun. 2015 PMID: 25758345