Human IL-33 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # D3300
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Cell Lysates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
1.65 pg/mL
Assay Range
6.25-400 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IL-33 Quantikine ELISA Kit
The Quantikine Human IL-33 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-33 in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant human IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-33. Results obtained using natural human IL-33 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-33.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-33
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Cell Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 34.1 | 112 | 214 | 36.4 | 116 | 224 |
| Standard Deviation | 1.21 | 3.22 | 5.36 | 2.8 | 6.3 | 10.4 |
| CV% | 3.5 | 2.9 | 2.5 | 7.7 | 5.4 | 4.6 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 34.3 | 111 | 218 | 39.0 | 120 | 230 |
| Standard Deviation | 1.45 | 3.33 | 5.00 | 2.2 | 5.6 | 12.8 |
| CV% | 4.2 | 3.0 | 2.3 | 5.6 | 4.7 | 5.6 |
Recovery for Human IL-33 Quantikine ELISA Kit
The recovery of human IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 81-106 |
| EDTA Plasma (n=4) | 86 | 82-92 |
| Heparin Plasma (n=4) | 84 | 80-90 |
| Serum (n=4) | 96 | 88-103 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-33 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human IL-33 Quantikine ELISA Kit
Human IL-33 ELISA Standard Curve
Human IL-33 ELISA Cell Culture Supernate/Cell Lysate Standard Curve
Human IL-33 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-33
Long Name
Interleukin 33
Alternate Names
C9orf26, DVS27, IL33, NF-HEV
Gene Symbol
IL33
Additional IL-33 Products
Product Documents for Human IL-33 Quantikine ELISA Kit
Product Specific Notices for Human IL-33 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.