Human IFN-alpha All Subtype Quantikine HS ELISA Kit

Name: Human IFN-alpha All Subtype Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HSFNAS0

R&D Systems, Inc. a Bio-Techne Brand

Catalog # HSFNAS0

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Key Product Details

Assay Length:	4.0 hours
Sample Type & Volume Required Per Well:	Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sample Types:	Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	0.17 pg/mL
Assay Range:	0.938 - 60 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Human IFN-alpha All Subtype Quantikine HS ELISA Kit Summary:	The Quantikine™ HS Human IFN-alpha All Subtype Immunoassay is a 4.0 hour solid phase ELISA designed to measure all human IFN-alpha subtypes in serum and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha subtypes.

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Product Specifications for Human IFN-alpha All Subtype Quantikine HS ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450 or 490nm (TMB or AP Substrate)
Reactivity	Human
Specificity	Natural and recombinant human IFN-alpha.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	7.68	14.7	29.1	8.1	15.8	31.3
Standard Deviation	0.175	0.377	0.665	0.357	0.728	1.7
CV%	2.3	2.6	2.3	4.4	4.6	5.4

Recovery

The recovery of human IFN-alpha spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	92	84-97
Heparin Plasma (n=4)	90	81-99
Serum (n=4)	93	85-104

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IFN-alpha were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IFN-alpha All Subtype Quantikine HS ELISA Kit

	Human IFN-alpha All SubType ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Item	Value
Long Name	Interferon alpha
Entrez Gene IDs	3440 (Human)
Alternate Names	IFN-alpha, IFN-alphaA, IFNA, IFNA2, IFNA2b, IFNalpha, INFA2, LeIF D, interferon alpha 2

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