Human Cathepsin V Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCATV0
Discontinued Product
DCATV0 has been discontinued.
View all Cathepsin V products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (50 µL)
Sensitivity
7.14 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human Cathepsin V Quantikine ELISA Kit
The Quantikine Human Cathepsin V Immunoassay is a 4.5 hour solid phase ELISA designed to measure Cathepsin V in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human Cathepsin V and antibodies raised against the recombinant factor. Natural human Cathepsin V showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human Cathepsin V.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Reactivity
Human
Specificity
Natural and recombinant human Cathepsin V. This assay measures pro- and active Cathepsin V and also recognizes Cathepsin V when complexed with Cystatin C, Fetuin A, or Kinninogen.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 143 | 461 | 981 | 142 | 427 | 884 |
| Standard Deviation | 6.1 | 21.9 | 49.8 | 6.4 | 20.5 | 50.6 |
| CV% | 4.3 | 4.7 | 5.1 | 4.5 | 4.8 | 5.7 |
Recovery for Human Cathepsin V Quantikine ELISA Kit
The recovery of Cathepsin V spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 99 | 94-109 |
| EDTA Plasma (n=4) | 97 | 91-111 |
| Heparin Plasma (n=4) | 91 | 85-99 |
| Serum (n=4) | 98 | 89-112 |
| Urine (n=4) | 93 | 84-98 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Cathepsin V were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Cathepsin V
Cathepsin V, also known as Cathepsin L2 and Cathepsin U, is a cysteine protease of the papain family. It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0. Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium.
Additional Cathepsin V Products
Product Documents for Human Cathepsin V Quantikine ELISA Kit
Product Specific Notices for Human Cathepsin V Quantikine ELISA Kit
For research use only
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