Sign In / Register
Mouse CD3+ T Cell Enrichment Column, Small, Pack of 5
Catalog # MTCC-5 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Loading...
Summary for Mouse CD3+ T Cell Enrichment Column, Small, Pack of 5
T Cell Enrichment Columns
 |
Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.
CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 45% and 65% of initial CD3+ cells loaded. The purity of recovered cells ranges from 81% to 88%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.
Features
- Available for human, mouse, and rat cells
- Purifies CD3+ T cells to 81 – 88%
- Based upon gravitational flow
- Simple procedure
- No specialized equipment required
Kit Contents
- Cell Selection Column
- 10X Column Buffer
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
Citations for Mouse CD3+ T Cell Enrichment Column, Small, Pack of 5 (15)
Showing
1
-
10 of
15 citations
Showing All
Filter By:
Search Title:
Citations are publications that use Bio-Techne products. Selected citations for Mouse CD3+ T Cell Enrichment Column, Small, Pack of 5 include:
-
Species: Mouse
Sample Types: T Cells
H Qi et al. (2020), Foxo3 Promotes the Differentiation and Function of Follicular Helper T Cells Cell Rep, 31(6):107621.
PMID: 32402289 -
Species: Mouse
Sample Types: Whole Cells
JH DeLong et al. (2018), Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1 J. Immunol., 0(0).
PMID: 29358280 -
Species: Mouse
Sample Types: Whole Cells
HR Kim et al. (2018), TAGLN2 polymerizes G-actin in a low ionic state but blocks Arp2/3-nucleated actin branching in physiological conditions Sci Rep, 8(1):5503.
PMID: 29615809 -
Species: Mouse
Sample Types: Whole Cells
VR Chowdhary et al. (2017), Concomitant Disruption of CD4 and CD8 Genes Facilitates the Development of Double Negative ?? TCR(+) Peripheral T Cells That Respond Robustly to Staphylococcal Superantigen J. Immunol., 0(0).
PMID: 28468970 -
Species: Mouse
Sample Types: Whole Tissue
SM Seki et al. (2017), Lineage-Specific Metabolic Properties and Vulnerabilities of T Cells in the Demyelinating Central Nervous System J. Immunol., 0(0).
PMID: 28507026 -
Species: Mouse
Sample Types: Whole Cells
Na B et al. (2015), TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse. J Cell Biol, 209(1):143-62.
PMID: 25869671 -
Species: Mouse
Sample Types: Whole Cells
Andrijauskaite K et al. (2015), IL-12 conditioning improves retrovirally mediated transduction efficiency of CD8+ T cells. Cancer Gene Ther, 22(7):360-7.
PMID: 26182912 -
Species: Mouse
Sample Types: Whole Cells
Lee-Chang et al. (2014), Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity. Blood, 124(9):1450-9.
PMID: 25037628 -
Species: Mouse
Sample Types: Whole Cells
Drees J et al. (2014), Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli. Protein Expr Purif, 94(0):60-6.
PMID: 24215864 -
Species: Mouse
Sample Types: Whole Cells
Xu W et al. (2013), Efficacy and mechanism-of-action of a novel superagonist interleukin-15: interleukin-15 receptor alphaSu/Fc fusion complex in syngeneic murine models of multiple myeloma. Cancer Res, 73(10):3075-86.
PMID: 23644531 -
Species: Mouse
Sample Types: Whole Cells
Stumhofer JS et al. (2010), A role for IL-27p28 as an antagonist of gp130-mediated signaling. Nat. Immunol., 11(12):1119-26.
PMID: 21057510 -
Species: Mouse
Sample Types: Whole Cells
Nath N et al. (2009), Loss of AMPK exacerbates experimental autoimmune encephalomyelitis disease severity. Biochem. Biophys. Res. Commun., 386(1):16-20.
PMID: 19486896 -
Species: Mouse
Sample Types: Whole Cells
Rubinstein MP et al. (2008), IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response. Blood, 112(9):3704-12.
PMID: 18689546 -
Species: Mouse
Sample Types: Whole Cells
Rodriguez-Manzanet R et al. (2008), TIM-4 expressed on APCs induces T cell expansion and survival. J. Immunol., 180(7):4706-13.
PMID: 18354194 -
Species: Mouse
Sample Types: Whole Cells
McKay D et al. (2006), Simultaneous deletion of MyD88 and Trif delays major histocompatibility and minor antigen mismatch allograft rejection. Eur. J. Immunol., 36(8):1994-2002.
PMID: 16874736
There are no citations that match your criteria.
