Mouse CD3+ T Cell Enrichment Column, Mini, Pack of 10 Summary
T Cell Enrichment Columns
Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.
CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 43% and 51% of initial CD3+ cells loaded. The purity of recovered cells ranges from 83% to 90%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.
Available for human, mouse, and rat cells
Purifies CD3+ T cells to 83 – 90%
Based upon gravitational flow
No specialized equipment required
Cell Selection Column
10X Column Buffer
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
Preparation & Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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