Sign In / RegisterMouse CD3+ T Cell Enrichment Column, Mini, Pack of 10
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Mouse CD3+ T Cell Enrichment Column, Mini, Pack of 10 Summary
T Cell Enrichment Columns
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Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.
CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 43% and 51% of initial CD3+ cells loaded. The purity of recovered cells ranges from 83% to 90%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.
Features
- Available for human, mouse, and rat cells
- Purifies CD3+ T cells to 83 – 90%
- Based upon gravitational flow
- Simple procedure
- No specialized equipment required
Kit Contents
- Cell Selection Column
- 10X Column Buffer
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
Citations (22)
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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD3+ T Cell Enrichment Column, Mini, Pack of 10 include:
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Species: Mouse
Sample Types: Whole Cells
BW Richmond et al. (2020), Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD Mucosal Immunol, 0(0):.
PMID: 32968197 -
Species: Mouse
Sample Types: Whole Cells
E Ragonnaud et al. (2019), Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis Cancer Res., 0(0):.
PMID: 31575547 -
Species: Mouse
Sample Types: Whole Cells
J Zhang et al. (2019), The mechanistic study behind suppression of GVHD while retaining GVL activities by myeloid-derived suppressor cells Leukemia, 0(0):.
PMID: 30737483 -
Species: Mouse
Sample Types: Whole Cells
HR Kim et al. (2018), T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells Nat Commun, 9(1):3630.
PMID: 30194420 -
Species: Mouse
Sample Types: Whole Cells
HR Kim et al. (2017), An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages Sci Rep, 7(1):8731.
PMID: 28821818 -
Species: Mouse
Sample Types: Whole Cells
(2016), A novel fusion of ALT-803 (IL-15 Superagonist) with an antibody demonstrates antigen-specific antitumor responses J Biol Chem, 0(0):.
PMID: 27650494 -
Species: Mouse
Sample Types: Whole Cells
Meagher C et al. (2007), CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses. Diabetes, 56(3):809-17.
PMID: 17327452 -
Species: Mouse
Sample Types: Whole Cells
Dixit VD et al. (2007), Ghrelin promotes thymopoiesis during aging. J. Clin. Invest., 117(10):2778-90.
PMID: 17823656 -
Species: Mouse
Sample Types: Whole Cells
Laurie C et al. (2007), CD4+ T cells from Copolymer-1 immunized mice protect dopaminergic neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease. J. Neuroimmunol., 183(1):60-8.
PMID: 17196666 -
Species: Mouse
Sample Types: Whole Cells
Aiello FB et al. (2007), IL-7 induces myelopoiesis and erythropoiesis. J. Immunol., 178(3):1553-63.
PMID: 17237404 -
Species: Mouse
Sample Types: Whole Cells
Pan PY et al. (2007), Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function. Blood, 111(1):219-28.
PMID: 17885078 -
Species: Mouse
Sample Types: Whole Cells
Onoyama I et al. (2007), Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesis. J. Exp. Med., 204(12):2875-88.
PMID: 17984302 -
Species: Mouse
Sample Types: Whole Cells
de la Fuente MA et al. (2006), 3BP2 deficiency impairs the response of B cells, but not T cells, to antigen receptor ligation. Mol. Cell. Biol., 26(14):5214-25.
PMID: 16809760 -
Species: Mouse
Sample Types: Whole Cells
Eun SY et al. (2006), Cutting edge: rho activation and actin polarization are dependent on plexin-A1 in dendritic cells. J. Immunol., 177(7):4271-5.
PMID: 16982860 -
Species: Mouse
Sample Types: Whole Cells
Hermann-Kleiter N et al. (2006), PKCtheta and PKA are antagonistic partners in the NF-AT transactivation pathway of primary mouse CD3+ T lymphocytes. Blood, 107(12):4841-8.
PMID: 16514061 -
Species: Human
Sample Types: Whole Cells
Andres A et al. (2005), Phylogenetic disparity influences the predominance of direct over indirect pathway of antigen presentation in islet xenotransplantation. Transplant. Proc., 37(1):463-5.
PMID: 15808677 -
Species: Mouse
Sample Types: Whole Cells
Zamiri P et al. (2005), Thrombospondin plays a vital role in the immune privilege of the eye. Invest. Ophthalmol. Vis. Sci., 46(3):908-19.
PMID: 15728547 -
Species: Mouse
Sample Types: Whole Cells
Li Y et al. (2004), SPAK kinase is a substrate and target of PKCtheta in T-cell receptor-induced AP-1 activation pathway. EMBO J., 23(5):1112-22.
PMID: 14988727 -
Species: Mouse
Sample Types: Whole Cells
Wang D et al. (2004), Immunopharmacological and antitumor effects of second-generation immunomodulatory oligonucleotides containing synthetic CpR motifs. Int. J. Oncol., 24(4):901-8.
PMID: 15010828 -
Species: Mouse
Sample Types: Whole Cells
Kipnis J et al. (2004), Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration. J. Neurosci., 24(27):6133-43.
PMID: 15240805 -
Species: Mouse
Sample Types: Whole Cells
Krautwald S et al. (2004), Ectopic expression of CCL19 impairs alloimmune response in mice. Immunology, 112(2):301-9.
PMID: 15147573 -
Species: Mouse
Sample Types: Whole Cells
Stolina M et al. (2003), Regulatory effects of osteoprotegerin on cellular and humoral immune responses. Clin. Immunol., 109(3):347-54.
PMID: 14697750
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