GlutaminePlus – 200mM (100X)

Name: GlutaminePlus – 200mM (100X)
Brand: R&D Systems
SKU: B90210

R&D Systems, part of Bio-Techne | Catalog # B90210

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Product Datasheet / COA / SDS

This item is in process of being discontinued.

Key Product Details

Features:

GlutaminePlus is an alternative to L-glutamine that can improve cell viability and growth. It is used as an effective substitute for L-glutamine in cell culture media.

Discontinued Product

B90210 has been discontinued. View all Cell Culture Media products.

Technical Data
Citations / Reviews

Summary for GlutaminePlus – 200mM (100X)

Why Use GlutaminePlus in Cell Culture?

L-glutamine is an essential amino acid required as an energy and carbon source by virtually all cells in culture. Exhaustion of L-glutamine in cell culture media has been shown to cause both reduced cell viability and productivity. Supplementation of media with L-glutamine products is frequently required to provide additional nutrients for optimal performance of cells in culture. GlutaminePlus is a stable L-glutamine product that offers a more consistent delivery of L-glutamine to cells in culture as compared to L-glutamine.

How is L-Glutamine Utilized for Cell Culture?

Normal L-glutamine has a relatively short half-life in solution, showing a temperature-dependent loss of product with time. L-glutamine degrades through an intramolecular cyclization reaction forming, as by-products, pyrrolidone carbonic acid and ammonia in equimolar concentrations. The rate of breakdown increases with increasing temperature and in the presence of phosphate or bicarbonate. Dipeptide derivatives of L-glutamine prevent the intramolecular cyclization reaction associated with solutions of L-glutamine. These derivatives are therefore stable in solution and allow the formulation of cell culture media containing L-glutamine that may be stored at 4°C for extended periods. Solutions containing these derivatives can even be autoclaved without appreciable degradation of the product (30 minutes at 121°C results in approximately <5% loss of the product). The dipeptide derivatives are metabolized within the cells to yield L-glutamine plus the second amino acid. This results in more consistent delivery of L-glutamine to cells in culture and avoids toxic buildup of ammonia in cell cultures. This feature can be especially important for ammonia sensitive cell lines.

Product Specifications for GlutaminePlus – 200mM (100X)

Applications	Cell Culture
Formulation	Liquid
Purification	0.2 μm filtered
Sterility Testing	Pass
Quality Testing	Pass

Preparation & Storage

Shipping Conditions	On Dry Ice
Storage	2 - 8 °C, protected from light

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GlutaminePlus – 200mM (100X)

R&D Systems, part of Bio-Techne | Catalog # B90210

Key Product Details

Summary for GlutaminePlus – 200mM (100X)

Why Use GlutaminePlus in Cell Culture?

How is L-Glutamine Utilized for Cell Culture?

Product Specifications for GlutaminePlus – 200mM (100X)

Preparation & Storage

Customer Reviews for GlutaminePlus – 200mM (100X)

Product Documents for GlutaminePlus – 200mM (100X)

Product Datasheets

COA

Certificate of Analysis

SDS

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