Around the angioreactor in the mouse, a capsule forms with time. When harvesting the angioreactor, it is difficult to dissect the capsule tissue from the opening of the angioreactor. There is a plug of tissue growing into the chamber. Is this normal? Do you have any recommendations on how to handle chambers like this?

Name: DIVAA FGF-2 VEGF
Brand: R&D Systems
SKU: 3450-048-B10

Growth of connective tissue into the angioreactor during angiogenesis is normal. The amount of connective tissue is dependent on the strain of mice, treatment, and response. The cell dissociation step may be increased up to 3 hours to improve dissociation/degradation, and any remaining connective tissue may be removed. If this does not solve the problem, it may be more appropriate to use the Optional Protocol for Dextran-FITC Detection (which measures capillary volume and discards cellular/connective tissue debris).

DIVAA FGF-2 VEGF

R&D Systems, part of Bio-Techne | Catalog # 3450-048-B10

Submit a review

Product Datasheet / COA / SDS

Key Product Details

Features:

DIVAA FGF-2 (1800ng)/ VEGF (600ng) is provided as an angiogenesis inducing growth factor cocktail for the Directed In Vivo Angiogenesis Assay (DIVAA).

Discontinued Product

3450-048-B10 has been discontinued. View all Angiogenesis Assay Kits products.

Technical Data
Citations / Reviews / FAQs

Summary for DIVAA FGF-2 VEGF

Why Use DIVAA FGF-2 (1.8 µg)/VEGF(600 ng)?

DIVAA FGF-2 (1800ng)/ VEGF (600ng) is provided in 60 µl at a concentration of 30 µg/mL FGF-2 and 10 µg/mL VEGF. This solution is diluted 20-fold when suspended in Cultrex RGF BME for a final concentration of 1.5 µg/mL FGF-2 and 500 ng/mL VEGF. Recombinant Mouse VEGF 164 is expressed by baculovirus, and recombinant FGF-2 is expressed in E. coli. FGF-2 / VEGF provides a stronger angiogenic response compared to the FGF-2 Positive Control for the Directed In Vivo Angiogenesis Assay (DIVAA).

Preparation & Storage

Stability	Product is stable for a minimum of 2 months from date of receipt when stored at -70 °C
Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Customer Reviews for DIVAA FGF-2 VEGF

There are currently no reviews for this product. Be the first to review DIVAA FGF-2 VEGF and earn rewards!

Have you used DIVAA FGF-2 VEGF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

DIVAA FGF-2 VEGF

R&D Systems, part of Bio-Techne | Catalog # 3450-048-B10

Key Product Details

Summary for DIVAA FGF-2 VEGF

Why Use DIVAA FGF-2 (1.8 µg)/VEGF(600 ng)?

Preparation & Storage

Customer Reviews for DIVAA FGF-2 VEGF

FAQs for DIVAA FGF-2 VEGF

Product Documents for DIVAA FGF-2 VEGF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

Stay Connected