Cultrex Mouse Collagen IV

Name: Cultrex Mouse Collagen IV
Brand: R&D Systems
SKU: 3410-010-02

R&D Systems, part of Bio-Techne | Catalog # 3410-010-02

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Product Datasheet / COA / SDS

2 vials (0.5 mg/vial) of Cultrex Mouse Collagen IV (Catalog # 3410-010-01)

Key Product Details

Features:	Cultrex Mouse Collagen IV is a purified stromal extracellular matrix protein that can promote cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture.
Key Benefits:	Qualified for general cell culture applications May be used as a hydrogel or thin coating for tissue culture vessels 1 mg of material is provided in 2 vials of Catalog # 3410-010-01 at 0.5 mg/vial

Technical Data
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Summary for Cultrex Mouse Collagen IV

Why Use Cultrex Mouse Collagen IV?

Collagen IV is a major component of basement membranes, which underlies epithelial and endothelial cells and surrounds muscle, fat and nerve cells. It can be used as a thin coating on tissue culture surfaces to promote cell attachment and proliferation and to study its effects on cell behavior.

Negative for the presence of bacteria and fungi.

Intended Use

Cultrex Mouse Collagen IV is used as a substrate for cell culture adhesion. The recommended working concentration is 1-10 μg/cm^2 (1-10 μg/mL) of growth surface depending on cell type. Cultrex Mouse Collagen IV (3410-010-02) is supplied as 2 vials of (3410-010-01) each containing 0.5 mg of protein.

Product Specifications for Cultrex Mouse Collagen IV

Species	Mouse
Source	Murine Engelbreth-Holm-Swarm (EHS) tumor
Endotoxin level	≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Tested for ability to promote attachment and spreading of rat pheochromocytoma (PC-12) cells.
Viral Testing	No mycoplasma contamination detected by PCR.

Preparation & Storage

Stability	Product is stable for 3 months from date of shipment when stored at ≤ -70 °C. See lot specifc Certificate of Analysis for expiration date.
Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Product is stable for 3 months from date of shipment when stored at -70 °C.

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Cultrex Mouse Collagen IV

R&D Systems, part of Bio-Techne | Catalog # 3410-010-02

Key Product Details

Summary for Cultrex Mouse Collagen IV

Why Use Cultrex Mouse Collagen IV?

Intended Use

Product Specifications for Cultrex Mouse Collagen IV

Preparation & Storage

Customer Reviews for Cultrex Mouse Collagen IV

Product Documents for Cultrex Mouse Collagen IV

Product Datasheets

COA

Certificate of Analysis

SDS

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