Cultrex HA-R-Spondin1-Fc 293T Cells
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Features: | Stably transfected 293T cells expressing murine R-Spondin 1 that have an N-terminal HA epitope tag and are fused to a C-terminal murine IgG2a Fc fragment. |
Citations for Cultrex HA-R-Spondin1-Fc 293T Cells (15)
Citations are publications that use Bio-Techne products. Selected citations for Cultrex HA-R-Spondin1-Fc 293T Cells include:
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Species: Human
Sample Types: Organoids
Applications: BioassayMM Capeling et al. (2022-01-01), Suspension culture promotes serosal mesothelial development in human intestinal organoids Cell Reports, 38(7):110379.
PMID: 35172130 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayAE Hall et al. (2022-01-01), RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer Nature Communications, 13(1):2791.
PMID: 35589755 -
Species: Human
Sample Types: Whole Cells
A Schwed-Gro et al. (2022-01-01), Glucocorticoids enhance chemotherapy-driven stress granule assembly and impair granule dynamics leading to cell death Journal of Cell Science, 0(0).
PMID: 35713120 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayYH Go et al. (2022-01-01), Modeling Pancreatic Cancer with Patient-Derived Organoids Integrating Cancer-Associated Fibroblasts Cancers, 14(9).
PMID: 35565206 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySK Chun et al. (2022-01-01), Disruption of the circadian clock drives Apc loss of heterozygosity to accelerate colorectal cancer Science Advances, 8(32):eabo2389.
PMID: 35947664 -
Species: Human
Sample Types: Whole Cells
GA Busslinger et al. (2021-01-01), Human gastrointestinal epithelia of the esophagus, stomach, and duodenum resolved at single-cell resolution Cell Reports, 34(10):108819.
PMID: 33691112 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayNP Tallapraga et al. (2021-01-01), Inflation-collapse dynamics drive patterning and morphogenesis in intestinal organoids Cell Stem Cell, 0(0).
PMID: 33915079 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayIA Bley et al. (2021-01-01), DKK1 inhibits canonical Wnt signaling in human papillomavirus-positive penile cancer cells Translational Oncology, 15(1):101267.
PMID: 34773828 -
N Kramer et al. (2020-01-01), Generation of Differentiating and Long-Living Intestinal Organoids Reflecting the Cellular Diversity of Canine Intestine Cells, 9(4).
PMID: 32231153 -
H Chen et al. (2020-01-01), Short-term organoid culture for drug sensitivity testing of high-grade serous carcinoma Gynecol. Oncol., 0(0).
PMID: 32253045 -
Species: Mouse
Sample Types: Bioassay
N McCarthy et al. (2020-01-01), Distinct Mesenchymal Cell Populations Generate the Essential Intestinal BMP Signaling Gradient Cell Stem Cell, 0(0).
PMID: 32084389 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Gao et al. (2019-01-01), Direct therapeutic targeting of immune checkpoint PD-1 in pancreatic cancer Br. J. Cancer, 120(1):88-96.
PMID: 30377341 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayY Yokoi et al. (2019-01-01), Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids Sci Rep, 9(1):2710.
PMID: 30804449 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayJH Jee et al. (2019-01-01), Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture Stem Cells Int, 2019(0):8472712.
PMID: 31312220 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayA Takakuwa et al. (2019-01-01), Butyric Acid and Leucine Induce alpha-Defensin Secretion from Small Intestinal Paneth Cells Nutrients, 11(11).
PMID: 31752111
There are no citations that match your criteria.
Customer Reviews for Cultrex HA-R-Spondin1-Fc 293T Cells (2)
Customer Images
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Verified Customer | Posted 12/28/2020For organoid culture
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Application: generation of organoids for in vivo studiesReason for Rating: Cells produced R-spondinVerified Customer | Posted 02/05/2019Used to confirm activation of Wnt signalling
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FAQs for Cultrex HA-R-Spondin1-Fc 293T Cells
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Murine R-Spondin 1 is reported to be a 27kDa protein, but the protein produced by the 293T cells in Catalog # 3710-001-01 is 70-75 kDa. Why?
The natural R-Spondin 1 protein is approximately 27 kDa. The cells in this product express a construct containing both an additional HA-tag plus an Fc fragment, hence the much larger size.
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For culturing and handling of the Cultrex HA-R-Spondin1-Fc 293T Cells (catalog # 3710-001-01), does the Fetal Bovine Serum need to be heat-inactivated in the Basal Growth Medium or Freeze Medium?
Heat-inactivated serum is not required for either of these media.
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Has this cell line been tested for mycoplasma?
Yes, all retail lots are tested to confirm mycoplasma is undetected by PCR, which is documented on the lot-specific CoA.