What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

How should Cultrex Basement Membrane Extract (BME) be stored and handled?

Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?

Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME. The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595; Corning 24-well, Catalog #3526; ThermoFisher 6-well, Catalog # 140675.

Cultrex Basement Membrane Extract, Type 2, Pathclear

Name: Cultrex Basement Membrane Extract, Type 2, Pathclear
Brand: R&D Systems
SKU: 3532-010-02
Price: 52 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3532-010-02

Citations (16)

Product Datasheet / COA

For Robust Organoid Cultures - Basement Membrane Extract

Try it on your cultures! Request a Sample of Cultrex BME, Type 2

Key Product Details

Features:	Cultrex Basement Membrane Extract (BME), Type 2 is specifically designed to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoid progenitor cells.
Key Benefits:	Qualified for use in organoid cell culture Commonly used robust and established organoid systems Quality controlled for peformance consistency

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Setting the standard in quality research reagents

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Citations (16) / Reviews / FAQs

Summary for Cultrex Basement Membrane Extract, Type 2, Pathclear

Why Use Cultrex BME, Type 2?

Cultrex Basement Membrane Extract (BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex RGF BME, Type 2 provides a proprietary formulation that is high in tensile strength and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.

Product Specifications for Cultrex Basement Membrane Extract, Type 2, Pathclear

Species	Mouse
Source	Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration	8-12 mg/mL as determined by Lowry assay
Endotoxin level	≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Organoid Culture - Cultrex BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells. Gelling Assay - Cultrex BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C. Dome Assay Cultrex BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates. Tube Formation Assay - Cultrex BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
Viral Testing	Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Preparation & Storage

Stability	Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Citations for Cultrex Basement Membrane Extract, Type 2, Pathclear (16)

Showing 1 - 10 of 16 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Cultrex Basement Membrane Extract, Type 2, Pathclear include:

Species: Transgenic Mouse
Sample Types: Organoid
Applications: Bioassay

EG Foerster et al. (2023), ATG16L1 protects from interferon-gamma-induced cell death in the small intestinal crypt Mucosal Immunology, 0(0).
PMID: 36792009
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Di Domizio et al. (2022), The cGAS-STING pathway drives type I IFN immunopathology in COVID-19 Nature, 0(0).
PMID: 35045565
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

H Daghero et al. (2022), Jejunum-derived NF-kappaB reporter organoids as 3D models for the study of TNF-alpha-induced inflammation Scientific Reports, 12(1):14425.
PMID: 36002565
Species: Human
Sample Types: Organoid
Applications: Bioassay

M Oro? et al. (2022), The molecular network of the proteasome machinery inhibition response is orchestrated by HSP70, revealing vulnerabilities in cancer cells Cell Reports, 40(13):111428.
PMID: 36170818
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

ZWM Tong et al. (2021), Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus Genes, 12(6).
PMID: 34200798
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MP Clark et al. (2021), Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models Cell Death Disease, 2021;12(7):641.
PMID: 34162831
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Kulsuptrak et al. (2021), A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection Cell Reports, 34(11):108859.
PMID: 33730579
Species: Human
Sample Types: Organoid

X Jin et al. (2020), LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer Mol. Cancer, 19(1):87.
PMID: 32393270
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Li et al. (2020), Breast cancer organoids from a patient with giant papillary carcinoma as a high-fidelity model Cancer Cell Int., 20(0):86.
PMID: 32206037
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KD Hulme et al. (2020), High glucose levels increase influenza-associated damage to the pulmonary epithelial-endothelial barrier Elife, 9(0).
PMID: 32697191
Species: Human
Sample Types: Organoids
Applications: Bioassay

B Pan et al. (2020), Establishment and characterization of breast cancer organoids from a patient with mammary Paget's disease Cancer Cell Int., 20(0):365.
PMID: 32774159
Species: Mouse
Sample Types: Organoids
Applications: Bioassay

P Francica et al. (2020), Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining Cell Rep, 32(8):108068.
PMID: 32846126
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

KD Hulme et al. (2020), High glucose levels increase influenza-associated damage to the pulmonary epithelial-endothelial barrier Elife, 9(0).
PMID: 32697191
Species: Mouse
Sample Types: Organoids

M Barazas et al. (2019), Radiosensitivity Is an Acquired Vulnerability of PARPi-Resistant BRCA1-Deficient Tumors Cancer Res., 79(3):452-460.
PMID: 30530501
Klueh U et al. (0), Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo. J Diabetes Sci Techno9(5):957-65.
PMID: 26306494
Okere B et al. (0), In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids. Int J Immunopathol Pharmaco28(3):390-402.
PMID: 26216908

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Showing 1 - 10 of 16 citations Showing All

FAQs for Cultrex Basement Membrane Extract, Type 2, Pathclear

What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex^® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
How does Cultrex^® Basement Membrane Extract (BME) promote cell differentiation?

All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
How should Cultrex Basement Membrane Extract (BME) be stored and handled?

Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.
What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?

Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME. The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595; Corning 24-well, Catalog #3526; ThermoFisher 6-well, Catalog # 140675.

Product Documents for Cultrex Basement Membrane Extract, Type 2, Pathclear

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