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Cultrex Basement Membrane Extract, PathClear

Basement Membrane Extract for use in multiple culture applications
Cultrex Basement Membrane Extract
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3432-001-01
3432-005-01
3432-010-01
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Assay Procedure

Citations (32)

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  • Cultrex PathClear Basement Membrane Extract (2 x 5 mL)
    Name: Anonymous
    Application: In vivo study
    Verified Customer | Posted 08/20/2018
  • Cultrex PathClear Basement Membrane Extract
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 04/01/2018
  • Cultrex PathClear Basement Membrane Extract
    Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 04/01/2018

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FAQs

  • What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  • How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  • How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

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