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Cultrex Basement Membrane Extract, PathClear, with Phenol Red Best Seller

Bio-Techne includes R&D Systems | Catalog # 3432-010-01P

Basement Membrane Extract for use in multiple culture applications

Key Product Details

Features: Cultrex Basement Membrane Extract (BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion.
Key Benefits:
  • Ideal for general cell culture application
  • Polymerizes at 37°C to form a reconstituted basement membrane
  • Quality controlled for peformance consistency
Cultrex Basement Membrane Extract (with Phenol Red)
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FAQs for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

  • What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  • How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  • How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

  • What is the percent of Laminin I in Cultrex® Basement Membrane Extract, PathClear, Catalog # 3432-010-01?

    Cultrex® Basement Membrane Extract (BME), Pathclear, is composed of ≤ 60 % of Laminin I. Laminin is the major component of Cultrex BME. Other components are Collagen IV, which makes up ≤ 40% and Entactin which makes up ≤ 10%.

Product Documents for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

Certificate of Analysis

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