Cultrex Basement Membrane Extract, PathClear, with Phenol Red
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
|Features:||Cultrex Basement Membrane Extract (BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion.|
Citations for Cultrex Basement Membrane Extract, PathClear, with Phenol Red
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
FAQs for Cultrex Basement Membrane Extract, PathClear, with Phenol Red
What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
How should Cultrex Basement Membrane Extract (BME) be stored and handled?
Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.
What is the percent of Laminin I in Cultrex® Basement Membrane Extract, PathClear, Catalog # 3432-010-01?
Cultrex® Basement Membrane Extract (BME), Pathclear, is composed of ≤ 60 % of Laminin I. Laminin is the major component of Cultrex BME. Other components are Collagen IV, which makes up ≤ 40% and Entactin which makes up ≤ 10%.