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Cultrex 3-D Culture Matrix Rat Collagen I

Rat Collagen I specifically qualified to support 3D culture applications.

Key Product Details

Features: 3-D Culture Matrix Collagen I is an extracellular matrix hydrogel that directs cells to grow in three dimensions and assemble into organotypic structures in vitro.
Cultrex 3-D Culture Matrix Rat Collagen I

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FAQs

  • What type of analysis is typically applied for organoid or 3-D cell cultures?

    Within the organoid, spheroid, or 3-D culture, cells may be assessed for morphology, apical/basal polarity, protein localization, and relative proliferation. In addition, cells may be isolated from the 3-D culture and evaluated for levels of RNA and protein expression, as well as modifications to DNA.

  • What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?

    Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.

  • What are 3-D cultures?

    3-D cultures are in vitro cultures where immortalized cell lines, primary cell lines, stem cells, or tissue explants are placed within hydrogel matrices, such as Cultrex Basement Membrane Extract, that mimic in vivo cell environments and allow cells to proliferate in three dimensions.

  • What is the advantage of 3-D culture over traditional 2-D culture?

    While 2-D culture has been used for studying many aspects of cell function and behavior, the tissue-culture treated plastic environment is unlike anything found within living organisms. As a result, cells in 2-D culture exhibit altered morphology, function, proliferation, and gene expression when compared to their emanating tissues. By placing these cells in a 3-D environment, they assume biological and biochemical characteristics similar to what is observed in vivo.

  • What are the variables associated with 3-D culture?

    The major variables associated with 3-D culture are cell type, cell seeding density, composition of hydrogel, thickness of hydrogel, stiffness of hydrogel, composition of cell culture medium, and time of culture.

  • What are the different types of 3-D culture?

    The two principal methods for performing 3-D culture are the top assay and embedded assay. For the top assay, cells are seeded on a thick gel of Cultrex Basement Membrane Extract (BME) or Extracellular Matrix Protein. A thin overlay of cell culture medium is then applied to the cells. For the embedded assay, cells are resuspended within a thick gel of Cultrex BME or ECM and the culture media is applied on top. The top assay is easier to setup, to control seeding densities, and to keep cells within one focal plane for analysis.

  • Which matrix should I use for 3-D culture?

    Choice of matrix should correspond to the environment that you wish to recapitulate. Cultrex Basement Membrane Extract (BME) will recapitulate the basal lamina, which underlie most cells of epithelial or endothelial origin. Cultrex Collagen I is the major constituent of connective tissue, and is commonly inhabited by stationary cells, such as fibrocytes and adipose cells, as well as migrating cells, such as mast cells, macrophages, monocytes, lymphocytes, plasma cells, and eosinophils.

  • How should cells be cultured prior to setting up the 3-D culture?

    Cells need to be healthy and actively dividing in 2-D culture. Cells should be passaged two or three times after resuspension from cryopreservation, and they should never surpass 80% confluency during each passage. Cells should also be assessed for viability using trypan blue, and they should exhibit less than 5% staining.

  • Will Cultrex 3-D culture Matrix rat Collagen 1, Catalog # 3447-020-01, still work if it has been frozen? 

    This product will likely not form a gel after being frozen.

  • Is Cultrex 3-D Culture Matrix Rat Collagen I (Catalog # 3447-020-01) atelocollagen or telocollagen?

    Cultrex 3-D Culture Matrix Rat Collagen I (Catalog # 3447-020-01) is telocollagen.

  • Can the Organoid Harvesting Solution, Catalog # 3700-100-01,  be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?

    The Organoid Harvesting Solution works well with BME (Basement Membrane Extract).  For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.

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