Can cells grown in Basement Membrane Extract (BME), such as Catalog # 3433-005-01, be used in Flow Cytometry?

Name: CellSperse
Brand: R&D Systems
SKU: 3450-048-05
Rating: 5 (1 reviews)

For preparation of a single cell suspension for applications such as FACS and re-culturing, digestion of the matrix and cell-cell bonds may be facilitated using enzymes such as Trypsin or Dispase. Our CellSperse, cat# 3450-048-05, is similar to Dispase, and can also be used for this purpose. However, some markers may be susceptible to degradation from CellSperse treatment. It is recommended to consult the literature for design of experiments for the particular cell type and markers of interest.

CellSperse

R&D Systems, part of Bio-Techne | Catalog # 3450-048-05

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Citations (2)

Product Datasheet / COA / SDS

Key Product Details

Features:

DIVAA CellSperse is provided as an enzymatic matrix and cell dissociation reagent for the Directed In Vivo Angiogenesis Assay (DIVAA).

Discontinued Product

3450-048-05 has been discontinued. View all Angiogenesis Assay Kits products.

Technical Data
Citations / Reviews (1) / FAQs

Summary for CellSperse

Why Use CellSperse?

DIVAA CellSperse is a neutral metalloprotease from Bacillus polymyxa that promotes BME digestion and gentle cell dissociation for angiogenesis quantification in the Directed In Vivo Angiogenesis Assay (DIVAA).

Preparation & Storage

Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

CellSperse

R&D Systems, part of Bio-Techne | Catalog # 3450-048-05

Key Product Details

Summary for CellSperse

Why Use CellSperse?

Preparation & Storage

FAQs for CellSperse

Product Documents for CellSperse

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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