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Proteome Profiler Rat XL Cytokine Array
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 1A: NR8383 normal rat lung cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure). |
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The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 1B: IEC-18 rat intestinal epithelial cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure). |
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The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 2A: Lysates from IEC-18 rat intestinal epithelial cells were left untreated or treated with 100 ng/mL PMA for 24 hours (200 μg lysate, 5 minute exposure. |
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The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 2B: Lysates from NR8383 normal rat lung cells were left untreated or treated with 5 μg/mL zymosan for 24 hours (200 μg lysate, 5 minute exposure). |
Citations for Proteome Profiler Rat XL Cytokine Array (9)
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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Rat XL Cytokine Array include:
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Species: Rat
Sample Types: Serum
S Halin Berg et al. (2022), Rat prostate tumors induce DNA synthesis in remote organs Scientific Reports, 12(1):7908.
PMID: 35551231 -
Species: Rat
Sample Types: Tissue Homogenates
QY Li et al. (2022), Neuroinflammation in the anterior cingulate cortex: the potential supraspinal mechanism underlying the mirror-image pain following motor fiber injury Journal of Neuroinflammation, 19(1):162.
PMID: 35725625 -
Species: Rat
Sample Types: Cell Culture Supernates
N Goshi et al. (2020), A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation J Neuroinflammation, 17(1):155.
PMID: 32393376 -
Species: Rat
Sample Types: Tissue Homogenates
M Li et al. (2020), Comparative study of intracavernous pressure and cavernous pathology after bilateral cavernous nerve crushing and resection in rats Asian J. Androl., 0(0).
PMID: 32235099 -
Species: Rat
Sample Types: Cell Culture Supernates
M Kersting et al. (2020), Subtoxic cell responses to silica particles with different size and shape Scientific Reports, 10(1):21591.
PMID: 33299057 -
Species: Rat
Sample Types: Cell Culture Supernates
MR Wu et al. (2020), Bidirectional Enhancement of Cell Proliferation Between Iron Oxide Nanoparticle-Labeled Mesenchymal Stem Cells and Choroid Plexus in a Cell-Based Therapy Model of Ischemic Stroke Int J Nanomedicine, 15(0):9181-9195.
PMID: 33239875 -
Species: Rat
Sample Types: Cell culture supernate
JS Heidel et al. (2020), The Effect of Cardiogenic Factors on Cardiac Mesenchymal Cell Anti-Fibrogenic Paracrine Signaling and Therapeutic Performance Theranostics, 10(4):1514-1530.
PMID: 32042319 -
Species: Rat
Sample Types: Platelet Microparticles
FF Chen et al. (2020), Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles Chin J Nat Med, 18(8):620-627.
PMID: 32768169 -
Species: Rat
Sample Types: Cell Culture Supernates
EM Hill et al. (2019), Dietary polyunsaturated fatty acids modulate adipose secretome and is associated with changes in mammary epithelial stem cell self-renewal J. Nutr. Biochem., 71(0):45-53.
PMID: 31272031
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Customer Reviews for Proteome Profiler Rat XL Cytokine Array (2)
FAQs for Proteome Profiler Rat XL Cytokine Array
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Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
