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The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 1A: NR8383 normal rat lung cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure). |
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The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 1B: IEC-18 rat intestinal epithelial cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure). |
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The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 2A: Lysates from IEC-18 rat intestinal epithelial cells were left untreated or treated with 100 ng/mL PMA for 24 hours (200 μg lysate, 5 minute exposure. |
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The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 2B: Lysates from NR8383 normal rat lung cells were left untreated or treated with 5 μg/mL zymosan for 24 hours (200 μg lysate, 5 minute exposure). |
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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Rat XL Cytokine Array include:
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Species: Rat
Sample Types: Cell Culture Supernates
N Goshi et al. (2020), A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation J Neuroinflammation, 17(1):155.
PMID: 32393376 -
Species: Rat
Sample Types: Tissue Homogenates
M Li et al. (2020), Comparative study of intracavernous pressure and cavernous pathology after bilateral cavernous nerve crushing and resection in rats Asian J. Androl., 0(0):.
PMID: 32235099
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Customer Reviews (2)
FAQs
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Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
