Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, serum, or cell lysate samples are diluted and incubated with each array part of the Non-Hematopoietic Panel. This panel is comprised of the Non-Hematopoietic Array and the Common Analytes Array, which is also used in the Hematopoietic Array Panel (R&D Systems, Catalog # ARY011). The membranes are washed to remove unbound material followed by incubation with their specific cocktail of biotinylated detection antibodies. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of receptor bound. Chemiluminescence is detected in the same manner as a Western blot.
2 Non-Hematopoeitic Array Membranes
2 Common Analyte Array Membranes
Antibody Detection Cocktail
Chemiluminescent Detection Reagents
Transparency Overlay Template
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
Simultaneously detect the levels of these soluble receptors in a single sample.
Common Analytes Array
Integrin alpha 6/CD49f
Integrin alpha 9
Integrin alpha V/CD51
IL-15 R alpha
Integrin beta 1/CD29
Integrin beta 2/CD18
Integrin beta 3/CD61
Integrin beta 4/CD104
Integrin beta 5
Integrin beta 6
CD23/Fc epsilon RII
Coagulation Factor II/Thrombin
Integrin alpha 3/CD49c
Integrin alpha 5/CD49e
Product Specifications for Proteome Profiler Human sReceptor Array, Non-hematopoietic
Preparation & Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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