Proteome Profiler Human Kidney Biomarker Array Kit

Kit Summary

To simultaneously detect the relative changes of 38 proteins in a single sample. No specialized equipment is necessary.

• Product Datasheet
• Troubleshooting Guide
• Assays for analytes represented in the Human Kidney Biomarker Array Kit
• Compatible with LI-COR* and chemiluminescence detection

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Urine, cell culture supernatant, and cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any biomarker/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of kidney biomarker bound. Chemiluminescence is detected in the same manner as a Western blot.

• 4 Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Wash Buffer
• Detection Antibody Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the Product Datasheet.

Stability and Storage

Store the unopened product at 2^° C to 8^° C. Do not use past expiration date.