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Proteome Profiler Human Kidney Biomarker Array Kit

Contains 4 membranes - each spotted in duplicate with 38 different kidney biomarker antibodies
Proteome Profiler Human Kidney Biomarker Array Kit
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ARY019

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FAQs

  • Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

  • Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?

    The proteinase inhibitors are recommended for cell and tissue lysates only.  You do not need them for cell culture supernates.

  • Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

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