Mouse Siglec-G APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7775A

R&D Systems, part of Bio-Techne
Discontinued Product
FAB7775A has been discontinued. View all Siglec-G products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 805903

View all Siglec-G Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Siglec-G
Glu20-Gln525
Accession # NP_766488

Specificity

Detects mouse Siglec-G in ELISA. In direct ELISA, no cross-reactivity with recombinant mouse Siglec-F or recombinant human Siglec-10 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Siglec-G APC-conjugated Antibody

Detection of Siglec-G antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of Siglec-G in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB1124P) and either (A) Rat Anti-Mouse Siglec-G APC-conjugated Monoclonal Antibody (Catalog # FAB7775A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Siglec-G APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-G

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by a V-type Ig-like domain which mediates sialic acid binding, followed by varying numbers of C2-type Ig-like domains. Mouse Siglec-G, the apparent ortholog of human Siglec-10, is a 110-120 kDa, 688 amino acid (aa) type I transmembrane protein mainly expressed on mouse B1-type B cells. It controls B1 cell survival, selection, expansion and calcium signaling by negatively regulating B cell receptor signals. A potentially secreted 269 aa variant diverges after the first two Ig-like domains. Mouse Siglec-G shares approximately 63% aa sequence identity with human Siglec-10 within the extracellular domain.

Long Name

Sialic Acid Binding Ig-like Lectin G

Alternate Names

Siglec-10, SiglecG

Entrez Gene IDs

243958 (Mouse)

Gene Symbol

SIGLECG

UniProt

NP_766488

Additional Siglec-G Products

Product Documents for Mouse Siglec-G APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Siglec-G APC-conjugated Antibody

For research use only

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