Mouse Carbonic Anhydrase IV/CA4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2414
Discontinued Product
MAB2414 has been discontinued.
View all Carbonic Anhydrase IV/CA4 products.
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 565112
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV
Glu18-Ser277
Accession # Q64444
Specificity
Detects mouse CA4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CA1, 2, 3, 4, 5A, 5B, 6, 7, 8, 9, 10, 11, 12, 13, 14, recombinant mouse CA9, 12, or 14 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Carbonic Anhydrase IV/CA4 Antibody
Carbonic Anhydrase IV in Mouse Small Intestine.
Carbonic Anhydrase IV was detected in perfusion fixed frozen sections of mouse small intestine using Mouse Carbonic Anhydrase IV Monoclonal Antibody (Catalog # MAB2414) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to nuclei, cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Carbonic Anhydrase IV in Mouse Kidney.
Carbonic Anhydrase IV was detected in perfusion fixed frozen sections of mouse kidney using Mouse Carbonic Anhydrase IV Monoclonal Antibody (Catalog # MAB2414) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Carbonic Anhydrase IV/CA4 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse small intestine and mouse kidney
Sample: Perfusion fixed frozen sections of mouse small intestine and mouse kidney
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase IV/CA4
Carbonic Anhydrase IV (CA4) is a 32 kDa GPI-anchored membrane zinc metalloenzyme that is involved in bicarbonate transport and pH regulation. CA4 is expressed on the luminal surfaces of renal proximal tubules and pulmonary and retinal capillaries. Loss of function mutation of CA4 function is associated with retinal degeneration. Mature mouse and human CA4 share 55% amino acid sequence identity.
Additional Carbonic Anhydrase IV/CA4 Products
Product Documents for Mouse Carbonic Anhydrase IV/CA4 Antibody
Product Specific Notices for Mouse Carbonic Anhydrase IV/CA4 Antibody
For research use only
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