Human TGF-beta 1 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC240A
Discontinued Product
IC240A has been discontinued.
View all TGF-beta 1 products.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 9016
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant TGF-beta 1 and latent TGF-beta 1
Specificity
Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TGF‑ beta3 and recombinant amphibian TGF‑ beta5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TGF-beta 1 APC-conjugated Antibody
Detection of TGF‑ beta1 in PC‑3 human prostate cancer cell line by Flow Cytometry.
PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TGF-beta 1 APC-conjugated Monoclonal Antibody (Catalog # IC240A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human TGF-beta 1 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: PC‑3 human prostate cancer cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Sample: PC‑3 human prostate cancer cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Reviewed Applications
Read 1 review rated 4 using IC240A in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TGF-beta 1
Long Name
Transforming Growth Factor beta 1
Alternate Names
TGF beta1, TGFB, TGFB1, TGFbeta 1
Gene Symbol
TGFB1
Additional TGF-beta 1 Products
Product Documents for Human TGF-beta 1 APC-conjugated Antibody
Product Specific Notices for Human TGF-beta 1 APC-conjugated Antibody
For research use only
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