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Human PSCA Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7636

R&D Systems, part of Bio-Techne
Discontinued Product
MAB7636 has been discontinued. View all PSCA products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 741009

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PSCA
Leu21-Ser95
Accession # O43653

Specificity

Detects human PSCA in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human PSCA Antibody

PSCA antibody in Human Prostate by Immunohistochemistry (IHC-P).

PSCA in Human Prostate.

PSCA was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human PSCA Monoclonal Antibody (Catalog # MAB7636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human PSCA Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSCA

PSCA (prostate stem cell antigen) is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein of the Thy-1/Ly-6 family. PSCA is expressed in differentiating cells and epithelial cells of the prostate, bladder, kidney, skin, esophagus, stomach and placenta. It may be up- or down-regulated in cancer and may promote or suppress the tumor, depending on location. Mature human PSCA (amino acids 21-95) shares approximately 65% amino acid sequence identity with mouse and rat PSCA.

Long Name

Prostate Stem Cell Antigen

Alternate Names

PRO232

Entrez Gene IDs

8000 (Human); 72373 (Mouse)

Gene Symbol

PSCA

UniProt

Additional PSCA Products

Product Documents for Human PSCA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PSCA Antibody

For research use only

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