Human IRF5 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC4508A
Discontinued Product
IC4508A has been discontinued.
View all IRF5 products.
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IRF5
Ala214-Thr395
Accession # NP_002191
Ala214-Thr395
Accession # NP_002191
Specificity
Detects human IRF5 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human IRF5 APC-conjugated Antibody
Detection of IRF5 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Sheep Anti-Human IRF5 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC4508A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human IRF5 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IRF5
IRF5 is a member of the IRF family of transcription factors, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF5, along with IRF7, are the key mediators of TLR signaling. IRF5 forms heterodimers with IRF3 both of which are necessary for interferon gene transcription. IRF5 knock out mice indicate that IRF5 is critical for induction of apoptosis.
Long Name
Interferon Regulatory Factor 5
Alternate Names
IBD14, interferon regulatory factor 5, IRF-5, SLEB10
Gene Symbol
IRF5
UniProt
Additional IRF5 Products
Product Documents for Human IRF5 APC-conjugated Antibody
Product Specific Notices for Human IRF5 APC-conjugated Antibody
For research use only
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