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Human IL-15R alpha Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1471N

R&D Systems, part of Bio-Techne
Discontinued Product
FAB1471N has been discontinued. An alternative/replacement product is available: FAB10900N. View all IL-15R alpha products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 151303

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-15 R alpha
Ile31-Thr172
Accession # EAW86418

Specificity

Detects human IL‑15 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rhIL‑2 R beta, rhIL‑2 R gamma, or rmIL‑15 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IL-15R alpha Alexa Fluor® 700-conjugated Antibody

Detection of IL-15 Ra antibody in Human CD4+PBMCs antibody by Flow Cytometry.

Detection of IL‑15 R alpha in Human CD4+PBMCs by Flow Cytometry.

Human CD4+peripheral blood mononuclear cells (PBMCs) treated with 1 µg/mL PHA for 5 days were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human IL-15 Ra Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1471N) or (B) Mouse IgG2BAlexa Fluor 700 Isotype Control (Catalog # IC0041N). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-15R alpha Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Human CD4+ peripheral blood mononuclear cells (PBMCs) treated with PHA

Reviewed Applications

Read 1 review rated 2 using FAB1471N in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-15R alpha

Interleukin 15 receptor alpha (IL-15 R alpha) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 aa protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gammac, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References

  1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
  3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
  4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Long Name

Interleukin 15 Receptor alpha

Alternate Names

CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA

Entrez Gene IDs

3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)

Gene Symbol

IL15RA

UniProt

Additional IL-15R alpha Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-15R alpha Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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