Human DLL1 Alexa Fluor® 594-conjugated Antibody

Name: Human DLL1 Alexa Fluor® 594-conjugated Antibody
Brand: R&D Systems
SKU: FAB1818T

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB1818T

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human DLL1 Ser22-Gly540 Accession # AAG09716
Applications:	Flow Cytometry
Label:	Alexa Fluor 594 (Excitation=590 nm, Emission=617 nm)
Specificity:	Detects recombinant human DLL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse DLL4 is observed.

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Applications for Human DLL1 Alexa Fluor® 594-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25-1 µg/10⁶ cells	T98G human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

Item	Value
References	Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. Karanu, F.N. et al. (2001) Blood 97:1960. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. Takahashi, Y. et al. (2003) Development 130:4259. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68. Kiernan, A.E. et al. (2005) Development 132:4353. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749. Hozumi, K. et al. (2004) Nat. Immunol. 5:638. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454. Limbourg, A. et al. (2007) Circ. Res. 100:363. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
Long Name	Delta-like 1
Entrez Gene IDs	28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names	DL1, DLL1, Delta, Delta 1, Delta1, Drosophila Delta homolog 1, H-Delta-1, delta (Drosophila)-like 1, delta-like 1 (Drosophila), delta-like protein 1

Product Specific Notices for Human DLL1 Alexa Fluor® 594-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human DLL1 Alexa Fluor® 594-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human DLL1 Alexa Fluor® 594-conjugated Antibody

Formulation, Preparation and Storage

Plan Your Experiments

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Background: DLL1

Product Specific Notices for Human DLL1 Alexa Fluor® 594-conjugated Antibody

Citations for Human DLL1 Alexa Fluor® 594-conjugated Antibody

Product Documents for Human DLL1 Alexa Fluor® 594-conjugated Antibody

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