FBXW11 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78060

Novus Biologicals, part of Bio-Techne
Discontinued Product
NBP1-78060 has been discontinued. View all FBXW11 products.

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FBXW11 Antibodies »

Product Specifications

Immunogen

This affinity purified FBXW11 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human FBXW11 protein. (Uniprot: Q9UKB1)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with FBXW11 from human, mouse, dog, bovine, chimpanzee, chicken and horse based on a 100% homology with the immunizing sequence. Cross-reactivity with FBXW11 from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for FBXW11 Antibody - BSA Free

Western Blot: FBXW11 Antibody [NBP1-78060]

Western Blot: FBXW11 Antibody [NBP1-78060]

Western Blot: FBXW11 Antibody [NBP1-78060] - This antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG diluted 1:20,000 in blocking buffer for 1 h at 4 C.

Applications for FBXW11 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000-1:100000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:200-1:1000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: FBXW11

FBXW11 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

Long Name

F-box and WD Repeat Domain Containing 11

Alternate Names

BTRC2, BTRCP2, Fbw11, FBXW1B, Hos, NEDJED

Entrez Gene IDs

23291 (Human)

Gene Symbol

FBXW11

UniProt

Q9UKB1 (Human)

Additional FBXW11 Products

Product Documents for FBXW11 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FBXW11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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