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Visualize Viral Pathogenesis

Before a vaccine can be generated and scaled, one must first understand the pathogen and its effect on the host. What is the pathogen’s life cycle in the host? What is the host’s immune response? Presumably the host’s immune response failed. Why?  This requires preclinical research using human samples. Together, Bio-Techne’s brands provide solutions for quantifying pathogen/host nucleic acid and protein changes during infections.

Antibodies

Localize and Visualize the Pathogen in the Host

Use antibodies to visualize the local context of the host during pathogen infection. Quality antibody products are built for reproducibility based on the 5 pillars of antibody validation: Genetic Strategy Validation, Orthogonal Validation, Independent Antibody Validation, Expression of Tagged Proteins Validation, and Biological Strategies Validation.

Use RNAScope® in situ hybridization to visualize pathogen RNA in the host. Compare pathogen load in infected tissue to that in uninfected tissues.

 

 

Protein

Understand Why the Immune System Fails

Understand why the immune system failed to respond appropriately to the pathogen using Immunoassays  and Tocriscreen Libraries.

Use cell culture as a  model system to understand host cell responses.

     Cell Culture Media

     Cell Expansion Kits

     Cell Selection Kits

Use biologically active proteins as immunogens to understand mechanisms of the host cell response.

Develop and Assess Vaccine Quality

What type of vaccine are you manufacturing? Cell culture is a useful tool for developing and assessing vaccine efficacy and safety. What type of immune response are you trying to stimulate? In addition to addressing these questions, one needs to choose clones with the ideal manufacturing characteristics such as robust cell growth and scalability.

GMP Proteins

Culture and Isolate the Vaccine

Culture antigen producing cells using GMP cytokines and growth factors. Transition to GMP proteins as early as possible for consistency as you develop your vaccine.

Create stable overexpressing cell lines using the TcBuster™ transposon delivery system. TcBuster has an insertion efficiency greater than 50%, improved viability and cellular expansion and no inadvertent transpositions.

Use Single-Cell Western Assays to verify monoclonality. Single cell western can also be used to evaluate isoform heterogeneity. Measure protein expresson in 1000 cells in 4 hours.

Micro-Flow Imaging (MFi) Particle Analysis Instrument by ProteinSimple

Vaccine Analytical Process Development

Use Simple Western and Maurice and iCE to assess purity, and identity of samples. Assess molecular weight to infer aggregation, fragmentation, degradation. Assess charge heterogeneity, a proxy for oxidation, glycation, deamidation, disulfide connectivity or translation fidelity.

Visualize polysaccharides with glycobiology enzymes via fluorescence.

iCE3

Vaccine Formulation Assessment and Stability Testing

Use Maurice and iCE to assess formulation. iCE can be used to quickly and accurately monitor charge variants, which is important for evaluating vaccine stability and efficacy. 

Use Micro-Flow Imaging (MFI) to quickly detect and distinguish subvisible particles. MFI can also be used for protein aggregation analysis, which is important for assessing vaccine stability. MFI  is more sensitive than traditional techniques and allows you distinguish between protein aggregates and and other particulates.  

Profile Immune Response to the Vaccine

Assess vaccine efficacy by visualizing pathogens in tissues collected from experimental animal model systems. Monitor the Immune response in animal models and human samples in order to evaluate vaccine utility and anticipate adverse immune responses to the vaccine.

Luminex Flexmap 3D instrument

Discover and Verify Immune Response to Antigen

Build the Luminex® assay that you need to detect up to 100 target analytes simultaneously.  

Use QuantikineTM ELISAs to validate one or two immune responses over the long term. Learn about other ELISA Kits in our portfolio.

Use RNAScope in situ hybridization to evaluate vaccine efficacy in animal model systems. Compare pathogen load in samples from vaccinated animals to that of unvaccinated animals.

 

Luminex is a registered trademark of Luminex Corporation.

Multianalyte Immunoassays Using Simple Plex for Neuroscience Research

Monitor the Immune Response in Real Time With Ella

Automated Simple Plex™ assays on the Ella™ platform have a total time to result of just 90 minutes. Factory calibrated multianalyte cartridges standardize your immunoassays, allowing you to reliably test for immune reactivity with a high level of precision and reproducibility. And with a four-log range of detection, you’ll pick up even very low pg/mL levels of endogenous analyte.

Find out how Simple Plex assays are used to expedite COVID-19 research!

Learn more about all of Bio-Techne's solutions for Coronavirus research!

Cell and Gene Therapy

Characterize Cellular Immunity

Characterize immune cells using flow cytometry or Single-Cell Western assays. Contact us to learn about our custom antibody conjugation on demand service service. We conjugate bulk as well as individual vials of retail antibodies.

Visualize immune cells using RNAscope multiplex in situ hybridization assays.

Profile the multi-antigen IgG response over time with Simple Western serology assays.

Vaccine Development Symposia and Webinars

Coronavirus Research Tools

New Research Tools to Help the Fight Against SARS-CoV-2

Bio-Techne aims to lead on the fight against COVID-19 by supporting SARS-CoV-2 research, to help the scientific community understand its mechanisms of infection and advance the development of effective therapies. Our bioactive cytokines and growth factors, immune cell reagents, and biologics instrumentation are all helping in the quest for therapies against this novel coronavirus.  In this symposium, Bio-Techne researchers will joined by guest speakers to discuss various exciting aspects of COVID-19 research.

 

cIEF and CE-SDS with iCE

Application of Next Generation Analytical Tools for Vaccine Development

Modern vaccine development relies on a wide range of technologies. Watch this webinar to learn how scientists from Merck, Medgene Labs and Bio-Techne leverage innovative and automated analytical technologies that support the entire vaccine development process, from discovery to QC and lot release.  In this webinar, we will provide concise technical overviews of relevant platforms and specific examples where these analytical tools fit in the vaccine development workflow.

Partner with Us to Develop and Scale Immunoassays

Custom Luminex Service

Custom Simple Plex Service

Custom ELISA Service

Custom Simple Western or Single-Cell Western Service

Cancer Biomarker Discovery