Webinar: Addressing the Challenges of Size and Purity Analysis for Fusion Proteins, ADCs and AAVs

Join our webinar on March 3 to discover the recent advancements in biotherapeutic size analysis and purity analysis using the Maurice CE‑SDS platform. Chia Thach (Bio‑Techne) will describe how the system improves workflows for characterizing Antibody‑Drug Conjugates (ADCs) and Adeno‑Associated Viruses (AAVs), highlighting how the Turbo CE‑SDS cartridge can significantly boost throughput and productivity. Caroline Daniels (Shattuck Labs) will demonstrate how her team uses the Maurice system to separate molecules based on differences in N‑linked glycosylation. After comparing the CE‑SDS Plus and Turbo cartridges for highly glycosylated heterodimeric fusion proteins, the Turbo CE-SDS cartridge was selected for routine use due to faster run times with maintained resolution. 

Advancing Charge Variant Characterization of ADCs and mAbs using icIEF Fractionation and LC-MS

Nicki Zhang (formerly MilliporeSigma) and Nelly Luong (Technical University of Braunschweig) discuss  integrated workflows to characterize mAbs and ADCs with varying levels of heterogeneity. Nicki demonstrates how icIEF fractionation combined with MS enhances the resolution and interpretability of charge variant profiles to support formulation development, process optimization, and comparability studies. Nelly describes a preparative fractionation method that improves the sensitivity for subsequent peptide mapping using LC-MS.

Explore recent publications to see the latest developments in CE Analysis using Maurice Platforms

(1) Determination of AAV Capsid Protein Ratios by Sodium Dodecyl Sulfate Capillary Electrophoresis (CE-SDS)—UV Absorption Method

Key Highlights:

• CE-SDS with UV detection determines AAV capsid protein (VP1, VP2, VP3) ratios by separating denatured viral proteins based on size, typically showing a ~1:1:10 stoichiometry. 
• Due to low capsid concentration in samples (ng/µL range), this method often requires specialized sample preparation to achieve necessary sensitivity. 
• CE-SDS provides automated, rapid, and quantitative analysis, offering better precision than traditional SDS-PAGE

(2) Stability Under Different Stress Treatments of a Virus-like Particle Vaccine Based on a Recombinant Hepatitis E Vaccine

Key Highlights:

• A recombinant VLP vaccine (Hecolin®) shows high stability under thermal (4-56°C), freeze-thaw, and mechanical stress, maintaining structural integrity and antigenicity.
• Studies established that the 20-30 nm particles, based on the p239 capsid protein, resist significant denaturation in accelerated studies, with stable conformation confirmed by differential scanning calorimetry (DSC) and surface plasmon resonance (SPR). 

In this episode from her series “Technical Tips from Monica Tyagi,” Dr. Tyagi discusses some simple but important steps for method development and optimization for the analysis of Antibody Drug Conjugates (ADCs), including utilizing the Maurice on-board mixing (OBM) feature and an optional 458 filter. She shares key resources highlighting icIEF + CE-SDS workflows. icIEF separates ADC isoforms based on isoelectric points, detecting charge variants that may arise from conjugation or degradation. CE-SDS separates ADC fragments based on size and can resolve light and heavy chains, aggregates, and fragments, providing purity profiles.