"Simple Western™ allows us to run three-hour assays, which would usually take us two days with traditional Western blots."
Laura Martins, Ph.D., Early Development Scientist, Oxford Biomedica
Abby re-imagines traditional Westerns with automated western blot technology. Simple Western assays on Abby are automated, capillary-based immunoassays that overcome complexities inherent to traditional Western. Abby automates Western blotting protocol steps such as antibody additions, incubations, and washes. Having problems detecting your protein of interest? No problem! Her picogram sensitivity and total protein detection, combined with fully analyzed results in 3 – 5 hours, ensure that you'll never want to touch a traditional Western blot again!
Chemiluminescent detection: Are you working with low abundance proteins or have little sample to spare for analysis? Abby solves both problems with her chemiluminescent detection. Enjoy picogram-level sensitivity that enables you to get the most data out of your samples.
Total Protein normalization. Reviewers are moving away from accepting normalization by housekeeping proteins due to their expression variability across different sample types. RePlex with Abby provides a more reliable method of normalizing to total protein.
Abby’s fully analyzed results can be viewed in either lane or electropherogram views. You can use the lane view option to compare band intensity or quantitatively analyze protein size and concentration. Compare the expression of proteins of interest to total protein. Data analysis with Compass’s user-friendly software is quick and easy. Save graph and baseline options with run files and quickly annotate lane view results with Compass lane view features. Lane annotation with Compass 6.0 saves hours spent annotating data in other programs. Spend more time thinking about your next paper and less time interpreting the results.
Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.
Reduce your lab time with automated simple western systems while accelerating your COVID-19 research. Set up, leave and remotely review your data in just 3 hours!
Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.
Quantitatively characterize serum or plasma human IgG antibodies reactive against 5 key viral antigens within one 3-hour run!
|Description||Total Protein Specification||Chemiluminescence Specification|
|Sample required||0.3-1.2 µg||0.6-1.2 µg|
|Volume required||3 µL/well|
|Size range||Molecular weight (MW) ladder ranges from 2-440 kDa|
|Resolution (± percent difference in MW)||
± 15-20% for MW <20 kDA
± 10% for MW >20 kDa
|Quantitation CV||<20% (total protein, chemiluminescence and fluorescence)|
|Dynamic range||2-3 logs||3-4 logs|
|Capillary||5 cm, 100 µm, 400 nL|
RePlex: 5 hours
|Samples per run||13 or 25|
|Dimensions (closed)||0.36 m H X 0.36 m W X 0.57 m D|
|Dimensions (open)||0.36 m H x 0.53 m W x 0.57 m D|
US/CAN 120 V AC, 60 hz, 4.2 amps
Europe 240 AC, 50 Hz, 2.1 amps
Japan 100 AC, 50/60 Hz, 5.0 amps
|Operating temperature||18-24 °C|
|Operating humidity||20-60% relative, non-condensing|