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Advanced Western Blotting Solutions for Targeted Protein Degradation & Drug Discovery

Simple Western™ provides you critical information to run quantitative DC50 curves with a high throughput workflow while providing you reproducible and quantifiable data. Simple Western assays and their automated Western blotting capabilities enhance accuracy, ensure data quality, and deliver results. Our systems integrate easily in PROTAC® screening assays while providing you the flexibility of seamless transferability of methods and an open platform.

Jess Instrument

Precise, Quantitative Data for the Accurate Determination of DC50 and Dmax Values

Boost your screening workflow with Simple Western systems, while running up to 96 samples in a single run! In this application note, 120 samples were screened in just two overnight runs to provide highly quantitative data which allows for an accurate determination of DC50 and Dmax. Here, off-the-shelf antibodies from Novus Biologicals and PROTAC® degraders from Tocris were used to automatically profile PROTAC®-induced Cereblon neosubstrate degradation.

 

Targeted Protein Degradation Graph

From Your Peers: Why Simple Western?

Simple Western on Jess Steps Up the Accuracy on Dose Response

"I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently."

-Rachel Doidge Ph.D., Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK

 

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Jess Breaks New Quantitative Ground in New Chemical Entity Screening at TCG Lifesciences

The quantitative assays and short run times on Jess enable us to generate data faster and be more confident in our results.”

-Tonmoy Sarkar, Ph.D., Senior Research Scientist, TCG Lifesciences

 

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Targeted Degradation of the Enhancer Lysine Acetyltransferases p300/CBP

Learn how dCBP-1 will be a useful tool for understanding p300/CBP activity at enhancers in both normal and diseased cells from Christopher J. Ott, PhD, Harvard Medical School.

 

Targeted Degradation

Quantitative Simple Western Assays for Targeted Protein Degradation Research

Are you wanting immunoassay-like quantitation for your Westerns with low sample volume? Get picogram-level sensitivity with just 3 µL of starting material! Learn how fully automated Simple Westerns are being used in labs around the world to quantify Degrader-induced protein knockdown. 

 

Simple Western Publications on Targeted Protein Degradation Research

 

Simple Westerns used for targeted protein degradation research article

Advanced Targeting Protein Degradation Research with Automated Western Blot

Watch Dr. Gary Allenby, CSO at Aurelia Bioscience, who shares how their research team is utilizing automated Western blot systems to rapidly and reproducibly quantify protein knockdown following treatment with Degraders. 

 

Advances in Targeted Protein Degradation

Validating Targets For Targeted Protein Degradation Using dTAG

dTAG degradation technology offers a generalizable strategy to degrade, in principle, any intracellular protein of interest. The broad applicability that it offers makes this a useful strategy for exploration and validation of targets, particularly in the context of new Degrader development programs.

 

Validating Targets for Targeted Protein Degradation using dTAG Whitepaper

Bio-Techne's Complete Solution for PROTAC® & Targeted Protein Degradation

The Bio-Techne family of brands offer a unique portfolio of high-quality reagents, instruments and services for researchers working in the rapidly growing field of Targeted Protein Degradation. Our TPD Research Product Guide highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation research including, Active Degraders, Degrader Building Blocks, Custom Degrader Services, Proteins and Assays for protein degradation. 

PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license

 

Bio-Techne’s complete targeted protein degradation solution

Monitoring Target Engagement in Drug Discovery

In drug discovery, confirmation of in-cell target engagement is a critical component of the drug development process. Confirming that a drug candidate engages its proposed target in the cell and determining the concentration at which it exerts the desired effect(s) fulfill fundamental criteria for translation to activity and efficacy in its target tissue. Thermal shift assays (TSA) are regularly used by industry and academia to uncover or confirm interactions using purified proteins. Recently, this type of assay has been adapted to a cellular format and is called the cellular thermal shift assay (CETSA). Importantly, by upgrading from a biochemical to a cellular system, biological relevance is preserved.

Dr. Matias Casás Selves, Research Scientist, Drug Discovery Program, Ontario Institute for Cancer Research, developed a CETSA-Simple Western assay to monitor protein stability during drug discovery. This application note highlights quantitative and reproducible CETSA data generated with Simple Western (CETSA-Simple Western). This assay verifies drug–target binding, and given its quantitative nature, the half maximal inhibitory concentration (IC50) is also calculated.

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Simple Western Applications

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Immuno-Oncology

 

Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples.

Solutions for Cell Signaling and Signal Pathways Analysis

Cell Signaling

 

Analyze entire signaling pathways with high sensitivity multiplexing and total protein normalization.

Testing for Lentivirus and AAVs

Cell and Gene Therapy

 

Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.

Advances in Targeted Protein Degradation

Targeted Degradation

 

Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.

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Pharmacokinetic Analysis

 

High-throughput pharmacokinetic analysis with quantitative, high-sensitivity assays directly in complex lysates and tissue homogenates. 

Exosomes

Exosomes

 

A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity Western blotting solutions.

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Vaccine Development

 

Improve reproducibility, quantitation, and time to market for your vaccine candidates.

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Neuroscience

 

Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.

Simple Plex HEK-HCP Assay

Bioprocess Impurities

 

Screen for processing-related impurities and host-cell related contaminants. Quantify empty/full capsid content of viral vectors like AAVs.

SARS-CoV-2

SARS-CoV-2 Research

 

Reduce your lab time while accelerating your COVID‑19 research. Set up, leave and remotely review your data in just 3 hours!

SARS-CoV-2 Serology

SARS-CoV-2 Serology Assay

 

Rapid quantitative characterization of human IgG antibodies in serum or plasma reactive against multiple SARS-CoV-2 antigens.