Key Product Details
Product Summary for Human SOX17 Antibody
Accession # Q9H6I2
Scientific Data Images for Human SOX17 Antibody
Detection of Human SOX17 by Western Blot.Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SOX17-regulated Genes by Chromatin Immunoprecipitation.Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR.
SOX17 in B16 Mouse Cell Line.SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Applications for Human SOX17 Antibody
Chromatin Immunoprecipitation (ChIP)
Sample: Endoderm-differentiated D3 mouse embryonic stem cell line chromatin, p21 promoter detected by standard PCR.
Sample: Immersion fixed B16 mouse melanoma cell line and immersion fixed endoderm differentiated BG01V human embryonic stem cells
Sample: Endoderm differentiated BG01V human embryonic stem cells and endoderm differentiated iBJ6 human induced pluripotent stem cell line
Sample: Endoderm differentiated BG01V human embryonic stem cells, SK‑OV‑3 human ovarian adenocarcinoma cell line and OVCAR‑3 human ovarian carcinoma cell line
Read 17 reviews rated 4.8 using AF1924 in the following applications:
- Immunocytochemistry/Immunofluorescence (10 Reviews)
- Immunofluorescence (3 Reviews)
- Immunohistochemistry (2 Reviews)
- Western Blot (1 Review)
- single cells from a ESC to endoderm i... (1 Review)
Read 291 publications using AF1924 in the following applications:
- Bioassay (1 Publication)
- Flow Cytometry (40 Publications)
- Functional Assay (1 Publication)
- ICC (191 Publications)
- IF (3 Publications)
- IHC (70 Publications)
- IHC-Fr (6 Publications)
- IHC-P (2 Publications)
- IHC/IF (1 Publication)
- Immunoprecipitation (1 Publication)
- Neutralization (1 Publication)
- Western Blot (14 Publications)
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).
- Kanai-Azuma, M. et al. (2002) Development 129:2367.
- Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
Entrez Gene IDs
Product Specific Notices for Human SOX17 Antibody
For research use only