Goat IgG HRP-conjugated Antibody Best Seller
Catalog # HAF017 | R&D Systems, Inc. a Bio-Techne Brand
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Antibody Source
Product Summary for Goat IgG HRP-conjugated Antibody
Immunogen
Specificity
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Host
Isotype
Scientific Data Images for Goat IgG HRP-conjugated Antibody
Detection of Goat IgG Primary Antibody by Western Blot
Western blot shows detection of Goat Anti-Human/Mouse/Rat Syntaxin 1A Polyclonal Antibody (Catalog # AF7237) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Goat IgG HRP-conjugated Antibody
Western Blot
Reviewed Applications
Read 11 reviews rated 5 using HAF017 in the following applications:
Published Applications
Read 59 publications using HAF017 in the following applications:
Formulation, Preparation and Storage
Purification
Formulation
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Stability & Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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Product Documents for Goat IgG HRP-conjugated Antibody
Product Specific Notices for Goat IgG HRP-conjugated Antibody
For research use only
Citations for Goat IgG HRP-conjugated Antibody (59)
Citations are publications that use Bio-Techne products. Selected citations for Goat IgG HRP-conjugated Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotDiez et al. (2023-06-30), Toxoplasma gondii inhibits the expression of autophagy-related genes through AKT-dependent inactivation of the transcription factor FOXO3a mBio
PMID: 37387601 -
Species: Mosquito
Sample Types: Tissue Homogenates
Applications: Western BlotWebb et al. (2023-06-12), Expression of anti-chikungunya single-domain antibodies in transgenic Aedes aegypti reduces vector competence for chikungunya virus and Mayaro virus Frontiers in microbiology
PMID: 37378291 -
Species: Bacteria
Sample Types: Whole Cells
Applications: ELISA DetectionBain et al. (2023-06-01), In vivo evolution of a Klebsiella pneumoniae capsule defect promotes complement-mediated opsono-phagocytosis and persistence during recurrent infection bioRxiv : the preprint server for biology
PMID: 37398264 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotTemponeras et al. (2023-05-03), Distinct modulation of cellular immunopeptidome by the allosteric regulatory site of ER aminopeptidase 1 European journal of immunology
PMID: 37134263 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotK Chan et al. (2022-12-30), Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors Heliyon, 2022-10(0):e12744.
PMID: 36597481 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotM Alannan et al. (2022-12-23), Targeting PCSK9 in Liver Cancer Cells Triggers Metabolic Exhaustion and Cell Death by Ferroptosis Cells, 2022-112(1).
PMID: 36611859 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotW Asanprakit et al. (2022-10-07), M1 macrophages evoke an increase in polymeric immunoglobulin receptor (PIGR) expression in MDA-MB468 breast cancer cells through secretion of interleukin-1beta Scientific Reports, 2022-112(1):16842.
PMID: 36207349 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotJS Fleck et al. (2022-10-05), Inferring and perturbing cell fate regulomes in human brain organoids Nature, 2022-10(0).
PMID: 36198796 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotM Pereira et al. (2022-08-16), The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways Cell Reports, 2022-040(7):111225.
PMID: 35977521 -
Species: Human
Sample Types: Seminal Plasma
Applications: Western BlotT Turunen et al. (2022-07-08), Seminal-Plasma-Mediated Effects on Sperm Performance in Humans Cells, 2022-011(14).
PMID: 35883590 -
Species: Canine
Sample Types: Cell Lysates
Applications: Western BlotK Marek et al. (2022-05-31), Persistent Infection of a Canine Histiocytic Sarcoma Cell Line with Attenuated Canine Distemper Virus Expressing Vasostatin or Granulocyte-Macrophage Colony-Stimulating Factor International Journal of Molecular Sciences, 2022-023(11).
PMID: 35682834 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotC Morel et al. (2022-04-06), Head-to-Head Study of Developmental Neurotoxicity and Resultant Phenotype in Rats: alpha-Hexabromocyclododecane versus Valproic Acid, a Recognized Model of Reference for Autism Spectrum Disorders Toxics, 2022-010(4).
PMID: 35448441 -
Species: Rat
Sample Types: Whole Cells
Applications: IFX Gao et al. (2022-03-24), Piceatannol suppresses inflammation and promotes apoptosis in rheumatoid arthritis?fibroblast?like synoviocytes by inhibiting the NF?kappaB and MAPK signaling pathways Molecular Medicine Reports, 2022-025(5).
PMID: 35322865 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotC Hu et al. (2022-02-09), Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias Nature, 2022-0602(7897):518-522.
PMID: 35140400 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotI Temponeras et al. (2022-02-08), ERAP2 Inhibition Induces Cell-Surface Presentation by MOLT-4 Leukemia Cancer Cells of Many Novel and Potentially Antigenic Peptides International Journal of Molecular Sciences, 2022-023(3).
PMID: 35163832 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotS Murthy et al. (2022-01-24), TREM-1 is required for enhanced OpZ-induced superoxide generation following priming Journal of leukocyte biology, 2022-00(0).
PMID: 35075692 -
Species: Mouse,Human
Sample Types: Whole Tissue
Applications: IHCF Yao et al. (2021-12-17), A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 2021-112(1):7333.
PMID: 34921145 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCY Deng et al. (2021-12-06), Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance Nature Communications, 2021-112(1):7041.
PMID: 34873175 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotX Sun et al. (2021-11-03), Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion Nature, 2021-10(0).
PMID: 34732895 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotG Galaris et al. (2021-08-19), Thresholds of Endoglin Expression in Endothelial Cells Explains Vascular Etiology in Hereditary Hemorrhagic Telangiectasia Type 1 International Journal of Molecular Sciences, 2021-022(16).
PMID: 34445652 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotJ Hyun et al. (2021-07-27), Morus alba Root Extract Induces the Anagen Phase in the Human Hair Follicle Dermal Papilla Cells Pharmaceutics, 2021-013(8).
PMID: 34452116 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotJ Zhou et al. (2021-07-10), IL-6/STAT3 signaling activation exacerbates high fructose-induced podocyte hypertrophy by ketohexokinase-A-mediated tristetraprolin down-regulation Cellular Signalling, 2021-086(0):110082.
PMID: 34252535 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotC Vetrei et al. (2021-06-08), Immunomodulatory mAbs as Tools to Investigate on Cis-Interaction of PD-1/PD-L1 on Tumor Cells and to Set Up Methods for Early Screening of Safe and Potent Combinatorial Treatments Cancers, 2021-013(12).
PMID: 34201082 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotYG Gete et al. (2021-06-04), Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS Aging Cell, 2021-00(0):e13388.
PMID: 34086398 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotZ Li et al. (2021-04-08), Vaccine delivery alerts innate immune systems for more immunogenic vaccination JCI Insight, 2021-00(0).
PMID: 33690222 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotY Nagai et al. (2021-03-29), Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies Biomolecules, 2021-011(4).
PMID: 33805466 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotM Ogoyama et al. (2021-01-27), LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases International Journal of Molecular Sciences, 2021-022(3).
PMID: 33513878 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western BlotKK Oo et al. (2021-01-14), Development of an engineered peptide antagonist against periostin to overcome doxorubicin resistance in breast cancer BMC Cancer, 2021-021(1):65.
PMID: 33446140 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotRS Najjar et al. (2020-12-24), Cornus officinalis var. koreana Kitam polyphenol extract decreases pro-inflammatory markers in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing Akt phosphorylation Journal of ethnopharmacology, 2020-10(0):113734.
PMID: 33359857 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotS Yangngam et al. (2020-09-23), High level of interleukin-33 in cancer cells and cancer-associated fibroblasts correlates with good prognosis and suppressed migration in cholangiocarcinoma J Cancer, 2020-011(22):6571-6581.
PMID: 33046978 -
Species: Mouse
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,Flow CytometryJ Chida et al. (2020-08-26), Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice PLoS Pathog., 2020-016(8):e1008823.
PMID: 32845931 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotL Tu et al. (2020-08-13), TGF-beta2 interfering oligonucleotides used as adjuvants for microbial vaccines J. Leukoc. Biol., 2020-00(0).
PMID: 32794350 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotB Ganesan et al. (2020-06-23), Clinical relevance of B7H3 expression in retinoblastoma Sci Rep, 2020-010(1):10185.
PMID: 32576886 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotX Li et al. (2020-05-22), Epidermal growth factor regulates the development of stem and progenitor Leydig cells in rats J. Cell. Mol. Med., 2020-00(0).
PMID: 32441057 -
Species: Mouse
Sample Types: Protein
Applications: Western BlotZ Ren et al. (2020-03-26), Anti?glycolipid disorder effect of epigallocatechin?3?gallate on high?fat diet and STZ?induced T2DM in mice Mol Med Rep, 2020-021(6):2475-2483.
PMID: 32236613 -
Species: Mouse
Sample Types: Cell Culture Lysates
Applications: Western BlotS Barbaux et al. (2020-03-24), Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion Sci Rep, 2020-010(1):5335.
PMID: 32210282 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western BlotKA Lloyd et al. (2020-01-28), Netazepide Inhibits Expression of Pappalysin 2 in Type-1 Gastric Neuroendocrine Tumors Cell Mol Gastroenterol Hepatol, 2020-00(0).
PMID: 32004755 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotIF Scheiber et al. (2019-06-28), Manganese Uptake by A549 Cells is Mediated by Both ZIP8 and ZIP14 Nutrients, 2019-011(7).
PMID: 31261654 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotVB Lê et al. (2019-04-09), Human metapneumovirus activates NOD-like receptor protein 3 inflammasome via its small hydrophobic protein which plays a detrimental role during infection in mice PLoS Pathog., 2019-015(4):e1007689.
PMID: 30964929 -
Species: Human
Sample Types: Serum
Applications: ELISA DevelopmentH Leeman et al. (2018-12-08), Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine Transl Oncol, 2018-112(3):397-403.
PMID: 30530187 -
Applications: Western BlotT Tamberg et al. (2018-11-08), Blocking the proteolytic activity of zymogen matriptase�with antibody-based inhibitors J. Biol. Chem., 2018-10(0).
PMID: 30409910 -
Species: Mouse
Sample Types: Whole Tissue
G Liu et al. (2018-09-03), Streptozotocin?induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization Mol Med Rep, 2018-00(0).
PMID: 30221697 -
Species: Canine
Sample Types: Whole Cells
Applications: Western BlotEC Bwalya et al. (2017-05-04), Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes PLoS ONE, 2017-012(5):e0177144.
PMID: 28472120 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotSC Sebag et al. (2017-02-09), Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma JCI Insight, 2017-02(3):e88297.
PMID: 28194433 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotApichart Suksamrarn (2016-11-12), Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy In Vivo, 2016-130(6):869-877.
PMID: 27815474 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotWanner et al. (2015-04-15), Reversible linkage of two distinct small molecule inhibitors of Myc generates a dimeric inhibitor with improved potency that is active in myc over-expressing cancer cell lines. PLoS ONE, 2015-010(4):e0121793.
PMID: 25875098 -
Species: Avian - Quail,Avian - Turkey,Chicken
Sample Types: Tissue Homogenates
Applications: Western BlotAhn J et al. (2015-04-13), Identification of the avian RBP7 gene as a new adipose-specific gene and RBP7 promoter-driven GFP expression in adipose tissue of transgenic quail. PLoS ONE, 2015-010(4):e0124768.
PMID: 25867079 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotXie W et al. (2015-03-17), Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation. PLoS ONE, 2015-010(3):e0119738.
PMID: 25780921 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PZhang X et al. (2014-11-21), Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up. Mod Pathol, 2014-128(5):654-61.
PMID: 25412842 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotZouk H et al. (2014-03-01), Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus. Clin Exp Immunol, 2014-0175(3):485-97.
PMID: 24237155 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotSebag S et al. (2013-01-29), Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells. J Biol Chem, 2013-0288(11):7875-84.
PMID: 23362270 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotKrishnan A et al. (2012-09-24), Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis. Lab Invest, 2012-092(12):1712-25.
PMID: 23007134 -
Sample Types: N/A
Applications: Western BlotWu Z et al. (2012-03-05), Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure. J. Biol. Chem., 2012-0287(19):15557-69.
PMID: 22393061 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PHerrero LJ et al. (2011-07-05), Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis. Proc. Natl. Acad. Sci. U.S.A., 2011-0108(29):12048-53.
PMID: 21730129 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotMeen AJ et al. (2010-11-12), Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. J. Biol. Chem., 2010-1286(4):2636-47.
PMID: 21075844 -
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-PNadworny PL et al. (2010-02-19), Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. J Inflamm (Lond), 2010-07(0):13.
PMID: 20170497 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotDing Q et al. (2008-12-12), Identification of macrophage inhibitory cytokine-1 in adipose tissue and its secretion as an adipokine by human adipocytes. Endocrinology, 2008-1150(4):1688-96.
PMID: 19074584 -
Species: Human
Sample Types: Plasma
Applications: Western BlotNitta H et al. (2008-09-01), Production of C5a by ASP, a serine protease released from Aeromonas sobria. J. Immunol., 2008-0181(5):3602-8.
PMID: 18714034 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotHuang X et al. (2007-10-01), ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis. Invest. Ophthalmol. Vis. Sci., 2007-148(10):4626-33.
PMID: 17898286
There are no citations that match your criteria.
Customer Reviews for Goat IgG HRP-conjugated Antibody (11)
Customer Images
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Application: ELISASample Tested: SerumSpecies: Mouse and mouse serumVerified Customer | Posted 10/23/2023
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Application: Western BlotSample Tested: Recombinant proteinSpecies: MouseVerified Customer | Posted 08/31/2023
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Application: Western BlotSample Tested: Mv1Lu mink lung epithelial cell lineSpecies: MinkVerified Customer | Posted 11/20/2019
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Application: Western BlotSample Tested: Recombinant proteinSpecies: HumanVerified Customer | Posted 10/07/2019
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Application: Simple WesternSample Tested: Liver tissue, Muscle tissue, Skin tissue and human liver cellSpecies: Human and PigVerified Customer | Posted 08/14/2019
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Application: Western BlotSample Tested: HCT-116 human colorectal carcinoma cell lineSpecies: HumanVerified Customer | Posted 03/20/2019
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Application: ELISASample Tested: SerumSpecies: GoatVerified Customer | Posted 05/01/2018
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Application: ELISASample Tested: Human recombinant antibodySpecies: HumanVerified Customer | Posted 10/23/2017
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Application: Western BlotSample Tested: Peripheral blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 03/24/2017Western blot analysis of Human PD-1 in PBMCs using Goat anti-Human PD-1 antibody followed by HRP-conjugated Rabbit anti-Goat secondary antibody (#HAF017) at 1:1,000 dilution in 3% BSA-PBS blocking buffer.
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Application: Western BlotSample Tested: Kidney tissue and Heart tissueSpecies: MouseVerified Customer | Posted 08/26/2016
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Application: Western BlotSample Tested: Cancer cell lysatesSpecies: RabbitVerified Customer | Posted 02/22/2016The HRP-conjugated antibody worked as it is supposed to be. No complain. Will buy the product in the future.
There are no reviews that match your criteria.