Proteome Profiler Mouse XL Cytokine Array Best Seller

Catalog # ARY028 | R&D Systems, Inc. a Bio-Techne Brand
Contains 4 membranes-each spotted in duplicate with 111 different cytokine antibodies

Key Product Details

Detection of selected mouse cytokines in Balb/3T3 Cell Line Lysate
(3)
Detection of Selected Mouse Cytokines in CMT-93 Cell Line Lysate.
Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Supernate.
Setting the standard in quality research reagents
  • Preparation & Storage

    • Preparation & Storage

    Shipping Conditions The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Storage Store the unopened product at 2 - 8 °C. Do not use past expiration date.

    Assay Procedure

    Refer to the product datasheet for complete product details.

    Briefly, relative expression levels of mouse cytokines and chemokines in samples can be determined using the following procedure:

    • Prepare membrane and incubate with prepared sample
    • Incubate the membrane with Detection Antibody Cocktail
    • Incubate the membrane array with Streptavidin-HRP
    • Develop the membrane array with Chemi Reagents 1 and 2
    • Expose the membrane array to autoradiography film

    View Graphic Procedure
     

     

    Kit Contents
    • Rectangular 4-Well Multi-dish
    • 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
    • Array Buffer 4
    • Array Buffer 6
    • Chemi Reagent 1
    • Chemi Reagent 2
    • Detection Antibody Cocktail, Mouse XL Cytokine Array
    • Streptavidin-HRP
    • Transparency Overlay Template
    • Wash Buffer Concentrate (25X)

     

    Other Supplies Required

    Reagents

    Materials

    • Pipettes and pipette tips
    • Gloves
    • Plastic container with the capacity to hold 50 mL (for washing the arrays)
    • Plastic transparent sheet protector (trimmed to 10 cm x 12 cm and open on three sides)
    • Plastic wrap
    • Absorbent lab wipes (KimWipes® or equivalent)
    • Paper towels
    • X-ray film (Kodak BioMax™ Light-1) or equivalent
    • Flat-tipped tweezers

    Equipment

    • Rocking platform shaker
    • Microcentrifuge
    • Autoradiography cassette
    • Film developer
    • Flatbed scanner with transparency adapter capable of transmission mode
    • Computer capable of running image analysis software and Microsoft Excel

    Other Supplies Required for Cell Lysate Samples

    • Phosphate-Buffered Saline (PBS)
    • Lysis buffer (1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 10% glycerol, 2 mM EDTA, 10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)

    Other Supplies Required for Tissue Lysate Samples

    • PBS with protease inhibitors (10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
    • Triton™ X-100

     

    Procedure Overview

    R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler™ Mouse XL Cytokine Array Kit, Panel A (Catalog # ARY028)

    Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.

    Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.

    Place each array membrane in a separate well of the 4-Well Multi-dish.

    Incubate for one hour on a rocking platform shaker.

    Place each array membrane in a separate well of the 4-Well Multi-dish

    Add 0.5 mL Array Buffer 4 to each sample.

    Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6.

    Add 0.5 mL Array Buffer 4 to each sample.

    Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples.

    Incubate overnight at 2 °C to 8 °C on a rocking platform.

    Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

    Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.

    Add 0.5 mL Array Buffer 4 to each sample.
    Add 30 µL of Detection Antibody Cocktail to 1.5 mL of Array Buffer 4/6 for each array.
    Add 0.5 mL Array Buffer 4 to each sample.

    Pipette 1.5 mL diluted Detection Antibody Cocktail into each well of the 4-Well Multi-dish.

    Add 0.5 mL Array Buffer 4 to each sample.

    Return each array membrane to the 4-Well Multi-dish containing diluted Detection Antibody Cocktail.

    Incubate for one hour on a rocking platform shaker.

    Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

    Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.

    Add 0.5 mL Array Buffer 4 to each sample.

    Add 2 mL of diluted Streptavidin-HRP to each well of the 4-Well Multi-dish.

    Place the array membrane in the diluted Streptavidin-HRP solution.

    Incubate for 30 minutes on a rocking platform shaker.

    Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

    Add 0.5 mL Array Buffer 4 to each sample.

    Place the array membrane on a plastic sheet protector.

    Pipette 1 mL of the prepared Chemi Reagent Mix evenly onto the membrane.

    Cover the membrane with the top sheet of the plastic protector.

    Incubate for 1 minute.

    Blot off excess Chemi Reagent Mix.

    Wrap the membrane and sheet protector in plastic wrap.

    Place the wrapped array membrane in an autoradiography film cassette and expose to X-ray film.

    Add 0.5 mL Array Buffer 4 to each sample.
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    • Citations for Proteome Profiler Mouse XL Cytokine Array (120)

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    Customer Reviews for Proteome Profiler Mouse XL Cytokine Array (32)

    4.9 out of 5
    32 customer ratings
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    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 06/29/2023
      I bought two boxes, and they're both great
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 06/21/2023
      easy to use kit
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Khuynh Nguyen
      Verified Customer | Posted 05/06/2023
      This product works very well
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Felipe Rodrigues
      Verified Customer | Posted 10/16/2022
      Conditioned medium of macrophages (36h) isolated from metastatic lungs.
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 08/28/2022
      This kit is really great!
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Steven Nottley
      Verified Customer | Posted 08/07/2022
      Secretome of 4T1 murine breast cancer cells.
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Felipe Rodrigues
      Verified Customer | Posted 08/07/2022
      Cytokine array of the secretome of MLE12 cells.
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Dong Hu
      Verified Customer | Posted 07/11/2022
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 12/02/2021
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 10/21/2021
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 09/16/2021
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Soheil Saeedi
      Verified Customer | Posted 08/10/2021
      We used the array kit for profiling inflammatory cytokines in plasma samples from mice. I trust that it works very well.
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 03/14/2021
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 09/17/2020
      Excellent antibody array to screen a multitude of cytokines, chemokines, and growth factors in one small strip of membrane. Readout is not a fluorescence so you don't need a special fluorescence reader. If you are doing Western blotting, you can do this easily.
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 02/12/2020
      Mouse breast tumor tissues
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 01/25/2020
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 01/06/2020
      Excellent array!!
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 06/06/2019
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 03/13/2019
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 12/05/2018
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 11/11/2018
      Excellent specificity and detection for large number of cytokines. Works perfectly with little optimization.
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Monica Perez
      Verified Customer | Posted 11/05/2018
      Pancreas lysate. 750ug protein/ membrane
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 07/18/2018
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Leslie Priddy
      Verified Customer | Posted 04/01/2018
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 03/22/2018
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 12/07/2017
      200 uL pooled serum from wild-type C57Bl/6 mice.5 min exposure time
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Jakub Zmajkovic
      Verified Customer | Posted 08/17/2017
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 07/17/2017
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 05/23/2017
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: James Beckett
      Verified Customer | Posted 11/28/2016
      150 uL of mouse serum
      Proteome Profiler Mouse XL Cytokine Array ARY028
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 09/30/2016
      This is a great kit. It provides excellent reliability and a large number of cytokines, chemokines to screening in a single assay. I strongly recommend it.
    • Proteome Profiler Mouse XL Cytokine Array
      Name: Anonymous
      Verified Customer | Posted 08/26/2016
      The sample was cell culture supernatant collected from MEF.
      Proteome Profiler Mouse XL Cytokine Array ARY028

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    FAQs for Proteome Profiler Mouse XL Cytokine Array

    • Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

      The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

    • Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

      We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

    Product Documents for Proteome Profiler Mouse XL Cytokine Array

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.