Mouse Pentraxin 2/SAP Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MPTX20
Key Product Details
Assay Length
4.5
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.368 ng/mL
Assay Range
1.56-100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Pentraxin 2/SAP Quantikine ELISA Kit
The Quantikine Mouse PTX2/SAP Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PTX2/SAP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse PTX2/SAP and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse PTX2/SAP showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse PTX2/SAP.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse PTX2/SAP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 4.11 | 16.0 | 33.6 | 3.95 | 15.7 | 34.5 |
| Standard Deviation | 0.22 | 0.57 | 1.33 | 0.33 | 0.76 | 1.59 |
| CV% | 5.4 | 3.6 | 4.0 | 8.4 | 4.8 | 4.6 |
Recovery for Mouse Pentraxin 2/SAP Quantikine ELISA Kit
The recovery of PTX2/SAP spiked into cell culture samples was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=6) | 97 | 81-112 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse PTX2/SAP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse Pentraxin 2/SAP Quantikine ELISA Kit
Mouse Pentraxin 2/SAP ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Pentraxin 2/SAP
Pentraxin or Pentraxin 2 (PTX2), also known as Serum Amyloid P component (SAP), is a secreted serum glycoprotein belonging to the pentraxin superfamily. Pentraxins have a characteristic pentagonal discoid arrangement of five non-covalently bound subunits and bind a variety of molecules in a calcium- dependent lectin-like manner through a pattern-recognition-binding site. PTX2 is a major acute-phase protein and may function as an opsonin for a variety of ligands. PTX2 also associates ubiquitously with all amyloid deposits implicated in diseases including Alzheimer's and prion diseases, type 2 diabetes and various systemic amyloidoses.
Long Name
Serum Amyloid P Component
Alternate Names
APCS, PTX2, SAP
Gene Symbol
APCS
Additional Pentraxin 2/SAP Products
Product Documents for Mouse Pentraxin 2/SAP Quantikine ELISA Kit
Product Specific Notices for Mouse Pentraxin 2/SAP Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.