Mouse CCL11/Eotaxin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MME00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Citrate Plasma (13 µL)
Sensitivity
3 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Product Summary for Mouse CCL11/Eotaxin Quantikine ELISA Kit
The Quantikine Mouse Eotaxin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Eotaxin in cell culture supernates, serum, and plasma. It contains recombinant mouse Eotaxin and antibodies raised against the recombinant protein. This immunoassay has been shown to quantitate the recombinant protein accurately. Results obtained using natural mouse Eotaxin showed dose curves that were parallel to the standard curves obtained using the E. coli-expressed kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Eotaxin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Eotaxin. This assay cross-reacts 0.062% with Human Eotaxin and recognizes Rat Eotaxin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 49 | 197 | 466 | 46 | 179 | 446 |
| Standard Deviation | 3.2 | 7.2 | 30 | 3.8 | 11.7 | 28.7 |
| CV% | 6.5 | 3.7 | 6.4 | 8.3 | 6.5 | 6.4 |
Recovery for Mouse CCL11/Eotaxin Quantikine ELISA Kit
The recovery of mouse Eotaxin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 96 | 82-103 |
| Citrate Plasma (n=5) | 97 | 81-114 |
| EDTA Plasma (n=5) | 100 | 83-118 |
| Serum (n=5) | 98 | 82-111 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse Eotaxin in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse CCL11/Eotaxin Quantikine ELISA Kit
Mouse CCL11/Eotaxin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL11/Eotaxin
CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.
Additional CCL11/Eotaxin Products
Product Documents for Mouse CCL11/Eotaxin Quantikine ELISA Kit
Product Specific Notices for Mouse CCL11/Eotaxin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.