Total MMP-2 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # MMP200
For human, mouse, rat, porcine, and canine samples.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.082 ng/mL
Assay Range
0.5-32 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Total MMP-2 Quantikine ELISA Kit
The Quantikine Total MMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MMP-2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse/rat pro-MMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural MMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MMP-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human, Mouse, Rat, Porcine, Canine
Specificity
Recombinant MMP-2 and natural human, mouse, rat, porcine, and canine active, pro-, and TIMP complexed MMP-2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 4.30 | 11.2 | 22.8 | 4.26 | 11.1 | 21.5 |
| Standard Deviation | 0.302 | 0.420 | 0.828 | 0.275 | 0.738 | 1.51 |
| CV% | 7.0 | 3.8 | 3.6 | 6.5 | 6.6 | 7.0 |
Recovery for Total MMP-2 Quantikine ELISA Kit
The recovery of Total MMP-2 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 90-105 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total MMP-2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Total MMP-2 Quantikine ELISA Kit
Human/Mouse/Rat/Porcine/Canine Total MMP-2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-2
MMP-2 (matrix metalloproteinase 2), also known as Gelatinase A, is upregulated at sites of tissue damage, inflammation, and in stromal cells surrounding the invading front of metastatic tumors. The latent proenzyme (proMMP-2) is activated by MT-MMPs, Thrombin, or activated Protein C. Mature MMP-2 activity is inhibited by alpha 2-Macroglobulin or TIMPs 1, 2, 3, and -4. TIMP-2 and -3 can also tether proMMP-2 into cell surface ternary complexes with MT-MMPs. MMP-2 degrades Gelatin (denatured Collagen) and type IV Collagen, the major component of basement membranes. It can also degrade the matrix proteins Collagens V, VII and X, Decorin, Elastin, and Fibronectin. MMP-2 processes and modulates the functions of many other vasoactive and proinflammatory molecules including Adrenomedullin, Big Endothelin-1, Calcitonin gene-related peptide, CCL7/MCP-3, CXCL12/SDF-1, Galectin-3, IGFBP-3, IL-1 beta, S100A8, and S100A9.
Long Name
Matrix Metalloproteinase 2
Alternate Names
Gelatinase A, MMP2
Gene Symbol
MMP2
Additional MMP-2 Products
Product Documents for Total MMP-2 Quantikine ELISA Kit
Product Specific Notices for Total MMP-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.