Mouse LDLR Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MLDLR0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.064 ng/mL
Assay Range
0.313-20 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse LDLR Quantikine ELISA Kit
The Quantikine Mouse LDL R Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LDL R levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse LDL R and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse LDL R accurately. Results obtained using natural mouse LDL R showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse LDL R.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse LDL R. This kit also recognizes PCSK9/LDL R Complex.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.000 | 2.133 | 7.265 | 1.015 | 2.136 | 7.466 |
| Standard Deviation | 0.053 | 0.097 | 0.355 | 0.083 | 0.148 | 0.428 |
| CV% | 5.3 | 4.5 | 4.9 | 8.2 | 6.9 | 5.7 |
Recovery for Mouse LDLR Quantikine ELISA Kit
The recovery of mouse LDL R spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 104 | 80-114 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse LDL R were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse LDLR Quantikine ELISA Kit
Mouse LDL R ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LDLR
LDLR (Low Density Lipoprotein Receptor) is a widely expressed cell surface scavenger receptor. LDLR binds ApoB and ApoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively. Hepatocyte LDLR is responsible for endocytosis and clearing of most plasma LDL cholesterol. At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDLR to the cell surface. Lack of LDLR expression or function causes familial hypercholesterolemia (FH). The protease PCSK9 can also cause increased plasma cholesterol by promoting LDLR degradation rather than recycling to the cell surface.
Long Name
Low Density Lipoprotein Receptor
Alternate Names
LDL R
Entrez Gene IDs
Gene Symbol
LDLR
Additional LDLR Products
Product Documents for Mouse LDLR Quantikine ELISA Kit
Product Specific Notices for Mouse LDLR Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.